摘要
[目的]分析不同浓度亚硒酸钠对羊肚菌菌丝体在不同培养基上生长的影响,筛选出最佳的培养浓度,掌握多种培养基的最适富硒配方,为开发富硒羊肚菌产品提供技术支撑.[方法]以六妹羊肚菌作为富硒载体,亚硒酸钠为硒源,进行制备试验,通过平板、原种和栽培种制备试验,系统分析在不同培养基上,不同硒浓度对羊肚菌菌丝的生长趋势,菌丝密度,菌丝形态的影响.[结果]在液体摇培中,硒浓度≤20 mg/L,菌丝形态无明显变化,且随浓度升高,对羊肚菌菌丝促生作用增强,且20 mg/L时干重最大;在母种培养基上,硒浓度≤20 mg/L,对羊肚菌的菌丝生长,菌丝密度,菌丝形态均没有影响;在原种培养基上,硒浓度≤20 mg/L,菌丝密度与菌丝形态无明显变化,且对羊肚菌的菌丝有促生作用,在10 mg/L时最强;在栽培种培养基上,硒浓度20 mg/L,对羊肚菌菌丝体的促生作用最强,菌丝密度与菌丝形态均无明显变化.[结论]以亚硒酸钠作为硒源,硒浓度在10～20 mg/L效果最好,可作为羊肚菌富硒栽培较为理想的浓度.
Abstract
[Objective]To analyze the effects of different selenium concentrations on Morchella myceli-um,and to master the optimal formula of selenium-enriched medium in the hope of providing technical sup-port for the further development of selenium-enriched products.[Methods]Morchella sextelatam was used as selenium enriched carrier and sodium selenite as selenium source and the effects of different selenium concen-trations on the growth trend,mycelial density and mycelial morphology of Morchella sextelatam on different media were systematically analyzed.[Results]In liquid shake culture,when the concentration of selenium≤20 mg/L,the mycelial morphology no obvious change,and the promoting effect on The mycelial growth of Morchella sextelatam with the increase of concentration,and the dry weight at 20 mg/L.The culture medium,selenium concentration≤20 mg/L had no effect on the mycelial growth,mycelial density and mycelial mor-phology of Morchella sextelatam.the original mediumthe concentration of selenium≤20 mg/L,there was no significant change in mycelial density and mycelial morphology,and it promoted the growth of Morchella sexte-latam,which was the strongest at 10 mg/L.On the cultivation mediumselenium concentration of 20 mg/L the strongest promoting effect on the mycelium of Morchella sextelatam,and no significant change in mycelium density and mycelium morphology.[Conclusion]Sodium selenite is the best selenium source for Morchella sextelatam cultivation when concentration is 10～20 mg/L.
基金项目
微生物资源保护与开发利用重点实验室开放项目(YLUKLM202001)
伊犁州重点研究与技术开发专项项目(YZ2023A05)
万方数据