Physiological and biochemical characteristics of dominant lactic acid bacteria and cellulolytic bacteria in sunflower By-Products
[Objective]This study aims to provide a basis for fermented feeds by sunflower by-products.[Methods]The dominant lactic acid bacteria and cellulolytic bacteria attached to the surface of sunflower by-products were isolated,extracted and identified,and the physiological and biochemical characteristics of the dominant bacteria were analyzed.[Results]3 strains of lactic acid bacteria and 4 strains of cellulolytic bacte-ria were isolated.The 3 strains of lactic acid bacteria obtained were Enterococcus mundtii.Among the 4 strains of cellulolytic bacteria,strains Z2 and Z13 were Bacillus velezensis,X14 was Bacillus aryabhattai,and X4 was Ba-cillus amyloliquefaciens.3 strains of lactic acid bacteria grew well under the conditions of 4,10,30 and 45℃,3%and6.5%NaCl,grew at pH3.5-9,and did not grow at pH3;among the4 strains of cellulolytic bacteri-a,the ratio of transparent circle diameter(D)/colony diameter(d)and enzyme activity in order of size were Bacillus amyloliquefaciens>Bacillus velezensis>Bacillus aryabhattai.[Conclusion]Enterococcus mundtii has strong salt tolerance and a wide range of temperature adaptation,but its acid-producing ability is signifi-cantly weaker than that of Lactobacillus plantarum.In the actual weight loss rate of the product,the effect of in-oculation with Bacillus amyloliquefaciens is the best.
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