Effects of 2,4-epibrassinolide on growth and related genes expression of watermelon seedlings under drought Stress
[Objective]To reveal the effects of exogenous 2,4-epibrassinolide(EBR)on the growth and related gene expression of watermelon seedlings under drought stress,so as to provide a theoretical basis for the study of watermelon drought resistance.[Methods]Watermelon variety Qihong 1 was taken as the experi-mental material by using 15%polyethylene glycol(PEG6000)to simulate drought stress,and then,three treat-ments were set up:CK(clear water),PEG and PEG + EBR,in which the concentration of EBR was 0.01 mg/L.Finally,the biomass,photosynthesis index,chlorophyll content,antioxidant enzyme activity and expres-sion of related genes of watermelon seedlings after different treatments were detected.[Results]Spraying exog-enous EBR could alleviate the inhibition and damage of drought stress on the growth and development of water-melon seedlings and also activate EBR signal transduction pathway and improve the drought tolerance of water-melon seedlings by increasing the expression of response genes.Compared with PEG stress,the plant height,root length,aboveground fresh weight and underground fresh weight of watermelon seedlings sprayed with exog-enous EBR increased by 2.41%,36.76%,1.88%and 3.42%respectively.Moreover,it also enhances vari-ous indicators of photosynthesis and the activities of antioxidant enzymes(POD and SOD).The expression of key response genes BRI1,BIN2,BES1 and DWF4 of Brassinolide(BRS)signal transduction pathway also changed to varying degrees,and the expression of drought related genes CDSP32 and MYB101 also changed significantly.[Conclusion]Exogenous EBR can effectively alleviate the damage caused by drought stress to watermelon seedlings,thus improving the drought resistance of watermelon seedlings.
国家科技期刊平台
NSTL
万方数据
