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辣椒种间杂交遗传特性

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[目的]杂交育种是提高辣椒产量,改善辣椒品质的主要途径之一,为创制更多育种资源,提高辣椒杂交育种效率,通过种间杂交,测定辣椒种间杂交后代主要果实性状,分析主要性状的遗传特性,筛选高品质辣椒,为杂交育种和种质资源改良提供参考依据。[方法]以不同栽培种辣椒为研究对象,通过种间杂交获得子代,采用Indel分子标记技术检测种间杂交的成功率,采用高效液相测定果实辣素含量,用紫外分光光度计检测色价,采用SPSS和Origin 2019 等软件分析辣椒果实性状、品质性状的遗传多样性和相关性。[结果]利用Indel标记对28 份辣椒资源的杂交F1 真实性进行鉴定,一年生辣椒均可与魔鬼椒(ZL-280)和灌木椒(HY-1)杂交成功,主要果实性状的遗传特性均表现了超显性效应、加性效益和显性效应。但与鸟椒(X-260)未杂交成功,可能存在种间杂交障碍。[结论]辣椒不同种间杂交存在一定的杂交特性,一年生辣椒可顺利与魔鬼椒和灌木椒杂交,杂交后代的主要果实性状变异幅度大。
Genetic properties of interspecific crosses in pepper
[Objective]Screen high-quality pepper,this study will take different cultivated pepper as the research object,through interspecific hybridization,determine the main fruit traits of F1 generation,analyze the genetic characteristics of main fruit traits in the hope of providing a reference for future cross breeding and germplasm resource improvement.[Methods]Improve the efficiency of pepper cross breeding,F1 was obtained through interspecific hybridization.The success rate of interspecific hybridization was detected by Indel molecu-lar marker technology.The content of capsaicin in the fruit was determined by HPLC and the color value was detected by UV spectrophotometer.Afterwards,the genetic diversity and correlation of all pepper fruit traits and quality traits were analyzed by SPSS and Origin 2019 software.[Results]The authenticity of F1 hybrids among 28 pepper resources was identified by using Indel markers.The results showed that all the annuals could suc-cessfully cross with devil pepper(ZL-280)and shrub pepper(HY-1),and the genetic characteristics of the main fruit traits showed super dominance effect,additive effect and dominant effect.However,it failed to cross with bird pepper(X-260),which might be due to interspecific hybridization obstacles.[Conclusion]From this study,it can be concluded that there are certain hybridization characteristics in different interspecific hy-bridization of pepper.Annual pepper can be successfully crossed with devil pepper and shrub pepper,and the variation range of main fruit characters of hybrid offspring is large.

pepperInDel markerbreedinghybridization detectiongermplasm resources

张国儒、唐亚萍、石林媛、袁雷、张勇、杨生保

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新疆农业科学院园艺作物研究所,乌鲁木齐 830091

新疆农业大学,乌鲁木齐 830052

昌吉市泰诺丰农业科技有限公司,新疆昌吉 831100

辣椒 Indel标记 育种 杂交检测 种质资源

新疆维吾尔自治区青年科学基金新疆农科院青年科技骨干创新能力培养项目国家自然科学基金现代农业产业技术体系建设项目新疆农科院重点培育项目Xinjiang Academy of Agricultural Sciences Youth Science and Technology Backbone Innovation Ability Training Project

2022D01B170xjnkq-202000332060680CARS-24-G-29xjkcpy-2022005xjnkq-202003

2024

新疆农业科学
新疆农业科学院 新疆农业大学 新疆农学会

新疆农业科学

CSTPCD北大核心
影响因子:0.698
ISSN:1001-4330
年,卷(期):2024.61(3)
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