[目的]分析新疆生产建设兵团第一师阿拉尔市库尔勒香梨园梨树腐烂病病原菌的种群分类，通过 6对保守基因序列，研究库尔勒香梨腐烂病病原菌序列之间相关性及差异性。[方法]在库尔勒香梨园内采集具有典型症状的腐烂病病样，经科赫氏法则验证获取腐烂病病原菌纯培养。采用CTAB法提取腐烂病病原菌基因组DNA，通过PCR扩增ITS、β-tubulin、EF-1α、ACT、LSU、RBP2 基因并测序，利用分子系统学分析不同基因片段的系统发育关系。[结果]在阿拉尔周边混交园内库尔勒香梨腐烂病菌种为 Valsa mali var。pyri(V。ambiens、Valsa mali)。种内差异与地理位置有关，国内与国外梨腐烂病分离株差异较明显。EF-1α、ACT、LSU、RBP2 在进化过程中更能够体现种间差异，系统发育树分支多，存在种间差异。[结论]新疆生产建设兵团第一师阿拉尔市库尔勒香梨腐烂病病原菌种群分布和种间差异性，库尔勒香梨园的腐烂病病原菌主要为Valsa mali var。pyri、Valsa sordida。新疆南疆绿洲特色果园种植模式，以杨树等为主防护林最为常见，存在着林果树木腐烂病交叉侵染的可能。
Population structure analysis of pathogen of pear valsa canker in Aral area of Xinjiang
[Objective]The purpose of this study is to investigate the population classification and differ-ence by 6 pairs of gene sequences of the pathogens from Aral City,Ist Oivision,Xinjiang Production and Con-straction Corps.[Methods]Typical disease samples were collected for purified pathogens by Koch postu-lates.The total DNA of pathogens was extracted following a standard cetyl-trimethyl ammonium bromide(CTAB)method,subsequently,the ITS,β-tubulin,EF-1α,ACT,LSU,and RBP2 genes were conducted by PCR amplification and DNA sequencing.[Results]Pathogenic species of korla pear rot in mixed gardens a-round Alar Valsa mali var.pyri(V.ambiens、Valsa mali).Species differences are related ro geographical toca-tion.There are significant differences in pear rot disease isolates between domestic and foreign and foreign countries.EF-1α、ACT、LSU、RBP2 is more able to reflect interspecific differences,and phylogenetic tree has multiple branches,resulting in interspecific differences.[Conclusion]The results showed that Valsa mali var.pyri and Valsa sordida were the main diseases in Korla pear.It was clear that the difference existed be-tween the species and population distribution of canker disease pathogen in Korl pear.The main shelterbelt such as poplar is the most common windbreak in Southern Xinjiang,maybe there is a cross infection of fruit trees and wood trees.
gene sequenceValsa mali var.pyriValsa sordidamolecular identification
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