Identification of new pathogens of alfalfa anthracnose(Colletotrichum liriopes)and preliminary study on biological characteristics
[Objective]This project aims to isolate an anthrax strain from alfalfa with the symptoms of an-thracnose,which would be slightly different from the previously reported Colletotrichum trifolii and C.americae borealis in morphology in order to clarify the pathogenicity,taxonomic status and biological characteristics of this strain through a series of studies.[Methods]Anthrax strain was obtained by tissue isolation,and its path-ogenicity was proved by Koch's rule and morphological observation combined with PCR amplification of poly-gene fragments was used to identify the pathogenic bacteria.Meanwhile,the effects of different culture media,carbon source,nitrogen source,temperature,pH value and light on the colony growth of anthrax strain were de-termined.[Results]A new pathogenic anthrax strain was isolated from alfalfa,and identified as Colletotrichum liriopes.The strain could grow under the conditions of 5-35℃and pH 4-11,and most of the test medium,ni-trogen source and carbon source conditions.[Conclusion]C.liriopes can infect alfalfa and cause anthrac-nose.The optimum growth temperature of C.liriopes is 28℃,the optimum nitrogen source and carbon source are yeast and soluble starch respectively,the optimum pH is 7,the lethal temperature of mycelium is 61℃,and the alternation of light and dark for 12 h is favorable to the growth of the colony.
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