Effects of different gene editing types on traits of FGF5 gene-edited sheep wool
[Objective]To study the effects of different gene editing types on wool traits in FGF5 gene-edited fine-wool sheep in the hope of providing a theoretical basis for subsequent studies on the genetic pa-rameters of wool traits in gene-edited fine-wool sheep.[Methods]In this study,data on traits such as wool length,wool straight length,wool fiber diameter and greasy fleece weight from 181 FGF5-year-old gene-ed-ited fine-wood sheep from the sheep breeding base of Xinjiang Academy of Animal Sciences were collected for analysis.[Results]The wool length of FGF5 gene editing fine wool sheep was 10.49 cm with a coefficient var-iation of 11.82%.Wool length and greasy fleece weight of FGF5-InDel and FGF5-HDR gene-edited fine-wool were extremely significant higher than that of the wild type(P<0.01).The wool length and wool straight length of-28 bp or-26 bp,-2 bp and replacement(c.61 C>T)were extremely significant longer than that of the wild type(P<0.01),and the greasy fleece weight at-28 bp or-26 bp was significantly higher than that of the wild type(P<0.05).The wool length and wool straight length of double-allele-edi-ted and single-allele-edited mutations of the-28 bp genotype had extremely significantly longer than those of thewild type(P<0.01).[Conclusion]Different gene editing types of FGF5 gene editing in fine wool sheep significantly increase the wool length and greasy fleece weight,which can be used to form a core population of gene-edited sheep with outstanding phenotypic traits,promote the selection and expansion of FGF5 gene-ed-ited sheep.
FGF5 gene-edited fine-wool sheepedit typewool lengthgreasy fleece weightgeno-typemutation type
国家科技期刊平台
NSTL
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