摘要
[目的]研究薄皮甜瓜'灰鼠'对盐(NaCl)胁迫的生理响应机制,为甜瓜栽培及示范推广提供理论依据.[方法]采用基质盆栽试验,分别观测其在100 mmol/L NaCl胁迫5d及胁迫20d后在幼苗生长、离子稳态、抗氧化酶系统及渗透调节物质的变化情况.[结果]盐胁迫抑制了幼苗生长,破坏了离子平衡及抗氧化系统.甜瓜株高、叶柄长、叶片数随盐胁迫时间的延长而显著降低,茎粗、叶柄粗、叶片厚度随盐胁迫时间的延长而显著增加.盐胁迫后Na+外排速度降低,Na+含量增加,K+外流流速增大,K+含量减少.丙二醛含量显著增加,SOD、POD、CAT酶活性先升高后降低;可溶性糖、可溶性蛋白、游离脯氨酸含量显著增加.[结论]盐胁迫下,薄皮甜瓜'灰鼠'通过限制自身生长,增加叶片Na+含量、减少K+含量,并提高抗氧化酶活性,积累更多的渗透调节物质缓解盐胁迫.
Abstract
[Objective]To explore the physiological response mechanism of thin-skinned melon'Huishu'to salt(NaCl)stress and provide theoretical basis for melon cultivation,demonstration and promo-tion.[Methods]In this study,a substrate pot experiment was conducted to observe the changes in seedling growth,ion homeostasis,antioxidant enzyme system,and osmoregulation substances after 100 mmol/L NaCl short-term stress(5 d)and long-term stress(20 d).[Results]Salt stress inhibited seedling growth,de-stroyed ion balance and antioxidant systems.The plant height,petiole length,and leaf number decreased sig-nificantly with the prolongation of salt stress time,while the diameter,petiole diameter,and leaf thickness in-creased significantly with the prolongation of salt stress time.After salt stress,the Na+efflux velocity de-creased,the Na+content increased,the K+outflow velocity increased,and the K+content decreased.The content of malondialdehyde increased significantly.The content of malondialdehyde significantly increased,and the activities of SOD,POD,and CAT enzymes first increased and then decreased;the content of soluble sugar,soluble protein,and free proline increased significantly.[Conclusion]Under salt stress,the thin-skinned melon'Huishu'responds to salt stress by limiting its own growth,increasing the Na+content and re-ducing the K+content of its leaves,improving the antioxidant enzyme system,and accumulating more os-moregulation substances.
基金项目
国家西甜瓜产业技术体系-特色甜瓜品种改良岗(CARS-25)
新疆农作物生物技术重点实验室开放课题(XJYS0302-2022-4)
新疆农业科学院科技创新重点培育专项(xjkcpy-2022006)
新疆维吾尔自治区西甜瓜产业技术体系()
新疆维吾尔自治区天池英才项目(CARS-25)
Xinjiang Uygur Autonomous Region of watermelon and melon industry technology system-Deputy chief()
Xinjiang Uygur Autonomous Region Tianchi Talent Project()
国家科技期刊平台
NSTL
万方数据