一株群体感应抑制活性链霉菌的筛选鉴定及其毒力因子代谢能力分析

扫码查看

原文链接

国家科技期刊平台
NETL
NSTL
万方数据

中文摘要：[目的]挖掘干旱区放线菌天然产物代谢潜力，筛选群体感应抑制活性的产物，研究其对梨火疫病原菌毒力因子代谢能力的影响。[方法]利用紫色杆菌(Chromobacterium violaceum 026，CV026)群体感应抑制活性筛选模型，对新疆库木塔格沙漠周边干旱地区土壤中分离的放线菌进行发酵，采用牛津杯法对其发酵液进行活性筛选;鉴定群体感应抑制活性菌株及验证其发酵液提取和活性，分析粗提液对欧文氏菌毒力因子代谢影响，并采用液质联用(liquid chromatography and mass spectrometry，LC-MS)方法检测菌株代谢产物活性物质，挑选相似结构化合物验证群体感应抑制活性。[结果]获得一株具有明显群体感应抑制活性菌株K-9，其与Streptomyces rubradiris strain NBRC 14000T相似性达到99。72%。该菌发酵粗提液具有明显的群体感应抑制活性，可有效降低紫色杆菌CV026 产紫色素和梨火疫病原菌的游动能力，并对梨火疫病原菌生物膜、胞外多糖和胞外酶等毒力因子产生具有显著抑制活性，呈明显的浓度依赖性;从链霉菌K-9 粗提液中得到 635种化合物，苯基丙烯酸、4-羟基-3 甲氧基苯甲酸等5 种明显群体感应抑制化合物。[结论]干旱区放线菌K-9 为Streptomyces rubradiris，其发酵代谢产物具有明显群体感应抑制活性，能显著抑制欧文氏菌毒力因子的产生，并发现了多种新型的群体感应抑制活性化合物。

外文标题：Screening and identification of a Streptomyces strain with quorum sensing inhibitory activity and effect of the crude extracts on virulence factors of Erwinia amylovora

外文摘要：[Objective]To explore the potential metabolisms for natural product compounds from actino-mycetes in the arid area and investigate effect of the quorum sensing inhibition on virulence factors in Erwinia amylovora.[Methods]Chromobacterium violaceum 026(CV026)was used for the screening model of the quorum sensing inhibitions,the fermentation of actinomycetes isolated from soils in arid areas around the Ku-mutag Desert was carried out,and the quorum sensing inhibitory activity of the fermentation broth was tested u-sing the Oxford Cup method.The strain was identified by the 16S rRNA gene sequence analysis,and effect of crude extracts from the broth on virulence factors of Erwinia amylovora was measured.In addition to that,liq-uid chromatography and mass spectrometry(LC-MS)was used to measure metabolites,and compounds with similar structure to reported quorum sensing inhibitors were selected to validate.[Results]Isolate K-9 was screened and identified to belong to the genus Streptomyces,which showed strong quorum sensing inhibitory ac-tivity and shared the highest similarities with Streptomyces rubradiris NBRC 14000T(99.72%).The crude ex-tracts of the broth effectively reduced the violacein production of CV026 and the swimming ability of Erwinia amylovora.Virulence factors of Erwinia amylovora such as biofilm,pyocyanin and rhamnolipid were inhabited significantly with the concentration-dependent interactions.A total of 635 compounds were identified from the crude extract by LC-MS,and 5 compounds with significant quorum sensing inhibitory activity were observed.[Conclusion]Streptomyces sp.K-9 is one potential new species isolated from arid areas,whose fermentation metabolites shows obvious quorum sensing inhibitory activity,and can inhibit virulence factors produced by Pseudomonas aeruginosa.Additionally,many kinds of novel quorum sensing inhibitors were obtained.

外文关键词：

quorum sensing inhibitionactinomycete from arid areaErwinia amylovoravirulence fac-tors

作者：

柏晓玉、朱丽英、包慧芳、江凌、朱静、顾美英、朱艳蕾、张志东

展开 >

作者单位：

新疆师范大学生命科学学院,乌鲁木齐 830054

新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐 830091

南京工业大学化学与分子工程学院,南京 211816

南京工业大学食品与轻工学院,南京 211816

展开 >

关键词：

群体感应抑制剂干旱地区放线菌欧文氏菌毒力因子

基金：

新疆维吾尔自治区青年科技拔尖人才项目青年科技创新人才培养新疆农业科学院科技创新重点培育专项国家自然科学基金项目新疆维吾尔自治区自然科学基金杰出青年基金

项目编号：

2022TSYCCX0067xjkcpy-2021002320600042022D01E19

出版年：

2024

DOI：

10.6048/j.issn.1001-4330.2024.04.026

新疆农业科学

新疆农业科学院新疆农业大学新疆农学会

新疆农业科学

CSTPCD北大核心

影响因子：0.698

ISSN：1001-4330

年,卷(期)：2024.61(4)