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原驼乳中乳酸菌的分离筛选及特性分析

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[目的]挖掘和获得原驼乳中的乳酸菌资源。[方法]采集新疆乌鲁木齐县近效南山周边原驼乳样品,采用平板稀释法分离菌株,观察比对16S rRNA基因序列测序和菌株形态学特性,分析菌株分类学地位,并检测菌株溶血性和有害代谢物质等食用安全性。[结果]获得 29 株乳酸菌,经鉴定其归属于Leuconostoc、Lenti-lactobacillus两个属的4 个种;菌株均可耐受 0。5%胆盐,5%NaCl,且具有较好耐高温、牛奶胨化、抗氧化和抑制细菌等特性;所有菌株均无溶血现象,不产生硝基还原酶;除T12 外,均利用氨基酸不产生物胺。[结论]从原驼乳中获得的乳酸菌具有良好的发酵特性和食品安全性。
Screening,identification and characteristics of Lactic acid bacteria from raw camel milk
[Objective]To explore lactic acid bacteria(LAB)resources in raw camel milk.[Methods]Raw camel milk samples were collected around Nanshan mountain in Urumqi,Xinjiang and the strains were i-solated by the plate dilution method.The 16S rRNA gene sequences and morphological observation were car-ried out to clarify the taxonomic status of the strains,and edible safety characteristics such as detecting hemol-ysis and harmful metabolites.[Results]Results showed that a total of 29 LAB strains were isolated and identi-fied,which belonged to 4 species in Leuconostoc and Lentilactobacillus;the tested strains could tolerate 0.5%bile salt,5%NaCl,and showed good high temperature resistance,milk peptonization,anti-oxidation,dif-ferent enzyme activities,and antibacterial properties.None of strains were positive for hemolysis and produce nitroreductase.No biogenic amines were produced from amino acids by strains except T12.[Conclusion]The LAB obtained from camel milk in this study has good fermentation characteristics and food safety.

raw camel milkLactic acid bacteriascreening

孙建、李雪、楚敏、顾美英、艾尼江·尔斯满、朱静、何齐、谭慧林、张志东

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新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室,乌鲁木齐 830091

新疆农业大学食品科学与药学学院,乌鲁木齐 830052

阿克苏地区食品安全检测中心,新疆阿克苏 843006

原驼乳 乳酸菌 分离筛选

新疆维吾尔自治区公益性科研院所基本科研业务经费项目新疆维吾尔自治区自然科学基金项目新疆维吾尔自治区自然科学基金项目新疆区域协同创新专项(上海合作组织科技伙伴计划)项目新疆农业科学院自主培育项目团队建设专项

KY20220342021D01F622021D030022020E01007nkyzztd-001

2024

新疆农业科学
新疆农业科学院 新疆农业大学 新疆农学会

新疆农业科学

CSTPCD北大核心
影响因子:0.698
ISSN:1001-4330
年,卷(期):2024.61(4)