RNA-seq-based mining and analysis of drought-related genes in barley seedlings
[Objective]To excavate the genes related to drought resistance in the hope of providing theo-retical support for analyzing the molecular drought resistance mechanism of barley and guiding the drought re-sistance breeding of barley.[Methods]Taking New beer No.6 as the test material,we applied transcriptome sequencing RNA-seq technology to sequence the inverted bilobed leaves of barley seedlings before and after drought stress,and real-time fluorescence quantitative RT-PCR was used to verify the functional genes.[Results](1)In this study,3835 differentially expressed genes were detected in the inverted bilobed of New beer 6 before and after drought stress by using RNA-seq,mainly genes encoded by ABC transporter proteins,ribosomal proteins,transcription factors,dehydrins,peroxidases,protein phosphatases,etc.The results showed that DEG was mainly enriched in starch and sucrose metabolism,transporter proteins,plant hormone signaling transduction,and protein phosphatases.(2)By KEGG metabolic pathway enrichment analysis,DEGs were mainly enriched in starch and sucrose metabolism,transporter proteins,phytohormone signaling,chaperones and folding catalysts,aminosugar and ribosugar metabolism,phenylalanine metabolism,taurine and hyposulphite metabolism,peroxisomes and other pathways.[Conclusion]Significant differences are shown in gene expression before and after drought stress in barley.The results of this study have laid the foun-dation for the excavation of key drought-resistant genes and for further analysis of drought-resistant mecha-nisms in barley(1592 up-regulated genes and 2243 down-regulated genes).
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