Pathogen identification and biological characteristics analysis of wheat snow rot caused by Microdochium nivale
[Objective]The study aims to investigate the species and biological characteristics of the causal agents of wheat snow mold disease in the main wheat-producing areas of Yili Kazak Autonomous Pre-fecture(Yili Prefecture),Xinjiang Uygur Autonomous Region,China,in order to provide scientific basis for the development of pesticides and the prevention and control of the disease.[Methods]The pathogen of win-ter wheat snow mold disease was isolated by tissue culture method and inoculated onto healthy wheat plants to verify its pathogenicity.The species of the pathogen was identified by combining its biological characteristics and ITS gene sequence analysis.The biological characteristics of the pathogen were studied by the method of growth rate.[Results]The isolates YLXF-1,YLXF-2,and YLXF-3 could infect healthy wheat and ex-hibit the same symptoms of disease in the field.Morphological and molecular biological identification results indicated that the pathogen was Microdochium nivale.The pathogen could grow at temperatures of 5-35℃,pH values of 3-12,under different light conditions,and on various test media,and could utilize most of the tested carbon and nitrogen sources,indicating strong environmental adaptability.The pathogen had faster my-celial growth at 20℃,pH 8,and 12 h of light-dark alternation culture on PDA medium,and utilized sucrose and beef extract as the better carbon and nitrogen sources,respectively.[Conclusion]The pathogen of wheat snow mold disease in the main production area of winter wheat in Yili,Xinjiang is M.nivale.The optimal growth temperature of the pathogen mycelium is 20℃,pH value is 8,light is 12 h of light and darkness alter-nation,culture medium is PDA,the best carbon source is sucrose,and the best nitrogen source is beef ex-tract.
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