[目的]比较两种酵母培养物及其代谢成分的差异,为指导酵母培养物的生产及应用提供参考.[方法]材料为1 种自研酵母培养物与达农威益康 XP酵母培养物,利用非靶标代谢组学UHPLC-QTOF-MS技术,分析比较二者代谢产物成分及差异.[结果](1)在二级类别注释下,正、负离子模式分别注释到 614、497个化合物,主要代谢类别为有机酸,核苷、核苷酸及其衍生物,氨基酸及其衍生物,两种酵母培养物均无特有代谢成分,只在含量上有显著(P<0.05)差异.(2)共 237 个差异代谢物在正离子模式检出,176 个显著(P<0.05)上调表达,61 个显著(P<0.05)下调表达;136 个差异代谢物在负离子模式检出,64 个显著(P<0.05)上调表达,72 个显著(P<0.05)下调表达.(3)差异代谢物的 KEGG(Kyoto Encyclopedia of Genes and Ge-nomes)富集分析主要集中在丙氨酸、天冬氨酸和谷氨酸等代谢通路.[结论]自研酵母培养物与达农威益康XP的代谢产物含量存在显著差异,但其发酵原料及工艺更简便,并含有多种具药理、生理作用的代谢成分,有潜在应用价值.
Analysis of the composition differences between two yeast cultures based on untargeted metabolomics
[Objective]This project aims to compare the metabolic components differences between the XP and experimental group yeast culture,the findings will provide a reference for guiding the production and application of yeast culture.[Methods]The untargeted metabolomics UHPLC-QTOF-MS technology was used to analyze and compare the composition and differences of the metabolic components between the 2 yeast cultures.[Results](1)Under the super classification annotation,a total of 614 and 497 compounds were noted in positive and negative ion modes respectively.The main metabolic classes were organic acids,nucleo-sides,nucleotides and their derivatives,amino acids and their derivatives and so on.There were no specific metabolic components in the two yeast cultures,only significant differences in content(P<0.05).(2)In the positive ion mode,237 differential metabolites were detected,of which 176 were significantly up-regulated(P<0.05)and 61 were significantly down-regulated(P<0.05)in expression.In the negative ion mode,136 differential metabolites were detected,of which 64 were significantly up-regulated(P<0.05)and 72 were significantly down-regulated(P<0.05)in expression.(3)The KEGG(Kyoto Encyclopedia of Genes and Genomes)enrichment pathway of differential metabolites mainly focused on alanine,aspartate and glutamate metabolism.[Conclusion]Due to different carbon and nitrogen sources and fermentation conditions,the yeast cultures have significant differences in the content of metabolites,but with their own advantages of the biologi-cal activity of main differential metabolites.
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