Comparative study on biochemical indexes in plasma of different breeds of pregnant horse under the condition of same dietary nutrient levels
[Objective]Study the changes of blood routine biochemical indexes of pregnant mares in the later stage of pregnancy and provide reference for the healthy breeding and scientific breeding of pregnant mares.[Methods]18 Ili horses,21 Kazakh horses,21 thoroughbred horses,21 hybrid horses(Ili horses)with average age(7.50±1.50 years),similar parity(3-4 fetuses),consistent fat and health status were selected for the experiment♀×Orlovma♂)19 horses.The varieties were taken as the unit and they were di-vided into four experimental groups.Under the same dietary nutrition level and management conditions,the feeding experiment was conducted for 90 days.At the end of the trial period,the blood of pregnant horses was collected from the fasting neck vein in the morning and the relevant indexes were measured.[Results](1)the total protein,triglyceride and low density lipoprotein in plasma of pure blood horses were 58.39%(P<0.05),44.01%(P<0.01)and 32.16%(P<0.01)higher than those of the other three breeds,respec-tively.(2)The total bilirubin in plasma of Ili horse,Kazak horse and pure blood horse was significantly higher than that of hybrid horse(P<0.01),increased by 84.51%,131.35%and 93.17%,respectively.The di-rect bilirubin of Ili horse and Kazak horse was higher than that of hybrid horse by 82.50%and 89.83%,re-spectively(P<0.05).(3)The glucose levels of Ili horse and thoroughbred horse were 106.93%and 148.18%higher than that of Kazakh horse respectively(P<0.01).[Conclusion]Under the same nutrition level and feeding and management conditions,due to the difference of breeds,the protein,liver function en-zyme activity and blood sugar level of pure blood horses during pregnancy are superior to those of other breeds.Compared with the other three breeds,hybrid horses have defects in liver function metabolism and blood glucose level during pregnancy.