Identification of chicken circMICAL2,tissue expression profile analysis and its functional prediction
[Objective]The purpose of this study is to verify the authenticity of chicken circular RNA(circMICAL2)and explore the function and regulation mechanism of circMICAL2 in chicken muscle growth and development.[Methods]Specific primers based on circMICAL2 circular sequences were designed by PCR amplification,and RNase R and actinomycin D treatments were performed to detect the endogenous sta-bility of circMICAL2.Eight tissue samples from heart,liver,lung,kidney,intestine,skin,chest muscle and leg muscles of 16-day-old and 180-day-old chickens(adult chickens)were collected,and the tissue ex-pression profile of circMICAL2 in different development periods was completed by real-time PCR(qRT-PCR);Bioinformatics was used to predict circMICAL2-targeted miRNA and mRNA,and to carry out GO function and KEGG pathway enrichment analysis.[Results]Chicken circMICAL2 really existed,and its cycli-zation stability was strong;circMICAL2 was widely expressed in all tissues of adults and chicks,and circMI-CAL2 was most highly expressed in their chest and leg muscles of adults and chicks,and the expression of cir-cMICAL2 in both adult leg and thorax muscles was significantly higher than that in chicks(P<0.05),indica-ting that circMICAL2 was closely related to the regulation of chicken muscle growth and development.circMI-CAL2 targets gga-miR-103-3p and gga-miR-130b-3p regulated 225 downstream potential target genes.The target genes of circMICAL2 were mainly enriched in TGF-β signaling pathway,MAPK signaling pathway,cell cycle and other related signaling pathways.[Conclusion]Chicken circMICAL2 really exists,and the expression of circMICAL2 plays an important role in regulating the growth of muscle during different growth and development periods.
circRNAmusclechickentissue expressiongrowth and development
国家科技期刊平台
NSTL
万方数据
