PRRSV HNHK3-2021毒株的ORF5和ORF7序列分析
Sequence analysis of ORF3 and ORF5 of PRRSV HNHK3-2021 strain isolated in Hainan
范悦轩 1马佳镁 1黄春媛 2郑佳馨 2陈素贞 1张艳 2刘光亮 3曹宗喜1
作者信息
- 1. 新疆农业大学动物医学学院,乌鲁木齐 830000;海南省农业科学院畜牧兽医研究所/海南省热带动物繁育与疫病研究重点实验室,海口 571100
- 2. 海南省农业科学院畜牧兽医研究所/海南省热带动物繁育与疫病研究重点实验室,海口 571100
- 3. 新疆农业大学动物医学学院,乌鲁木齐 830000;海南省农业科学院畜牧兽医研究所海南省院士团队创新中心,海口 571100
- 折叠
摘要
[目的]研究海南猪繁殖与呼吸综合征病毒PRRSV的流行病学特征和遗传变异规律.[方法]采集海南省海口市及周边地区样品,分离出PRRSV HNHK3-2021 毒株,并测定NSP2 基因以及 ORF5 和ORF7 基因序列,采用Lasergene 7.1 进行序列比对分析,用 MEGA-X 进行遗传演化分析.[结果]分离出的 PRRSV HNHK3-2021 株为美洲型毒株,PRRSV HNHK3-2021 株和EF112447 HEB1、EU109503 GD、EU624117 XH-GD的ORF5 和ORF7 的核苷酸相似性较高,分别为99.2%～99.3%和 99.5%～100%,推导氨基酸相似性为99%～99.5%和 100%;PRRSV HNHK3-2021 株和 EU864232 SHB、AY262352_HB-2(sh)2002、AY656990 Abst-1、AY032626 CH-1a、EU807840 CH-1R 的 ORF5 和 ORF7 的核苷酸相似性为 87.1%～96.4%和91.4%～97.3%,推导氨基酸的相似性为 86.6%～93%和 91.9%～97.6%;PRRSV HNHK3-2021 株和JN654459 NADC30、KP860909 FJZ03、MH068878 SD17-38、AY881994 FJ-1 的ORF5 和ORF7 的核苷酸相似性为85.4%～87.1%和 89%～91.4%.氨基酸的相似性为 86.1%～87.1%和 89.5%～91.9%.[结论]RRSV HNHK3-2021 毒株和EU624117 XH-GD具有最近的遗传距离.
Abstract
[Objective]To explore the epidemiological characteristics and genetic variation of PRRSV in Hainan.[Methods]Samples were collected from Haikou and surrounding areas.The PRRSV strain HNHK3-2021 was isolated,and the NSP2 genes,as well as the ORF5 and ORF7 gene sequences,were deter-mined.Sequence alignment analysis was conducted using Lasergene 7.1,and genetic evolution analysis was performed using MEGA-X.[Results]The PRRSV HNHK3-2021 strain isolated was found to be of Ameri-can origin with high nucleotide similarity to EF112447 HEB1,EU109503 GD,and EU624117 XH-GD ORF5 and ORF7(99.2%-99.3%and 99.5%-100%,respectively).The derived amino acid similarity was 99%-99.5%and 100%.The nucleotide similarities of ORF5 and ORF7 of PRRSV HNHK3-2021 strain and EU864232 SHB,AY262352_HB-2(sh)2002,AY656990 Abst-1,AY032626 CH-1a,EU807840 CH-1R were found to be 87.1%-96.4%and 91.4%-97.3%,respectively.Similarly,the similarity of amino acids was 86.6%-93%and 91.9%-97.6%.The nucleotide similarities of ORF5 and ORF7 of PRRSV HNHK3-2021 strain and JN654459 NADC30,KP860909 FJZ03,MH068878 SD17-38,AY881994 FJ-1 were found to be 85.4%-87.1%and 89%-91.4%.The similarity of amino acids was 86.1%-87.1%and 89.5%-91.9%.[Conclusion]The PRRSV HNHK3-2021 strain and EU624117 XH-GD have the closest genetic distance.
关键词
猪繁殖与呼吸综合征/遗传进化/ORF5/ORF7Key words
porcine reproductive and respiratory syndrome/genetic evolution analysis/ORF5/ORF7引用本文复制引用
基金项目
海南省省属科研院所技术创新专项(jscx202001)
国家自然科学基金项目(32060796)
家畜疫病病原生物学国家重点实验室开放基金(SKLVEB2020KFKT021)
海南省农业科学院院本级科研项目(HAAS2022PT0205)
国家重点研发计划(2023YFC3404302)
出版年
2024
国家科技期刊平台
NSTL
万方数据