Sequence analysis of ORF3 and ORF5 of PRRSV HNHK3-2021 strain isolated in Hainan
[Objective]To explore the epidemiological characteristics and genetic variation of PRRSV in Hainan.[Methods]Samples were collected from Haikou and surrounding areas.The PRRSV strain HNHK3-2021 was isolated,and the NSP2 genes,as well as the ORF5 and ORF7 gene sequences,were deter-mined.Sequence alignment analysis was conducted using Lasergene 7.1,and genetic evolution analysis was performed using MEGA-X.[Results]The PRRSV HNHK3-2021 strain isolated was found to be of Ameri-can origin with high nucleotide similarity to EF112447 HEB1,EU109503 GD,and EU624117 XH-GD ORF5 and ORF7(99.2%-99.3%and 99.5%-100%,respectively).The derived amino acid similarity was 99%-99.5%and 100%.The nucleotide similarities of ORF5 and ORF7 of PRRSV HNHK3-2021 strain and EU864232 SHB,AY262352_HB-2(sh)2002,AY656990 Abst-1,AY032626 CH-1a,EU807840 CH-1R were found to be 87.1%-96.4%and 91.4%-97.3%,respectively.Similarly,the similarity of amino acids was 86.6%-93%and 91.9%-97.6%.The nucleotide similarities of ORF5 and ORF7 of PRRSV HNHK3-2021 strain and JN654459 NADC30,KP860909 FJZ03,MH068878 SD17-38,AY881994 FJ-1 were found to be 85.4%-87.1%and 89%-91.4%.The similarity of amino acids was 86.1%-87.1%and 89.5%-91.9%.[Conclusion]The PRRSV HNHK3-2021 strain and EU624117 XH-GD have the closest genetic distance.
porcine reproductive and respiratory syndromegenetic evolution analysisORF5ORF7