Functional analysis of 4-coumarate:CoA ligase gene Gh4CL30 in upland cotton
[Objective]To preliminarily study the biological function of 4-coumarate-CoA ligase(4CL)family gene gh4cl30,which might provide theoretical basis and genetic resources for cotton plant breeding.[Methods]Virus-induced gene silencing technology and gene editing technology was used to ob-tain gh4cl30-silenced and edited plants and determine the flavone and lignin contents of the gene suppressed and knockout plants to investigate field phenotype,seed size and fiber quality.[Results]Both gene silencing and gene editing of the gene resulted in dwarfing of the plants and a significant reduction of lignin content in the plant stalks.Field phenotypic measurements showed that seed size and fiber length were significantly re-duced in the gene edited plants.[Conclusion]Gh4CL30 affects cotton growth and development mainly through regulating lignin biosynthesis.
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