Extraction and decolorization of polysaccharides from fruit body of wild Agaricus balchaschensis in Xinjiang
[Objective]To study the extraction and decolorization process of crude polysaccharides from fruit body of wild Agaricus balchaschensis in Xinjiang,and provide theoretical basis and technical support for its in-depth development.[Methods]This study adopted ethanol subsiding to extract the fruit body polysac-charides of wild A.balchaschensisin,and explored the influence of extraction temperature,time and frequency on the yield of polysaccharides from A.balchaschensisin.With the decolorization time,decolorization tempera-ture,content of activated carbon and H2O2 as influencing factors and the decolorization rate of polysaccharides as research index,the study optimized the decolorization process of polysaccharides from A.balchaschensisin through orthogonal test,so as to determine the optimal decolorization conditions.[Results]According to the results,the optimal conditions for extracting fruit body polysaccharides of wild A.balchaschensis by ethanol subsiding were 6 h extraction time,60℃extraction temperature,and 2 times extraction,under which the yield of fruit body polysaccharides of wild A.balchaschensisin was 15.6%.The optimal decolorization process for ac-tivated carbon was 25%activated carbon,60℃decolorization temperature,and 45 min decolorization time at pH3,under which the decolorization rate was 80.4%.The optimal decolorization process for hydrogen perox-ide was 10%H2O2 content,60℃decolorization temperature,and 3 h decolorization time at pH 9,under which the decolorization rate was 86.23%.[Conclusion]This technology has verified the feasibility and practical value of applying ethanol subsiding to extract fruit body polysaccharides of wild A.balchaschensis in and the effects of H2O2 and activated carbon decolorization methods on the decolorization of polysaccharides from A.balchaschensis varied greatly under different process conditions.The former has a higher decolorization rate than the latter and is feasible for the decolorization of polysaccharides from A.balchaschensisin.
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