犊牛腹泻症的病原学检测及鉴定
Etiological detection and identification of calf diarrhea
苗书魁 1王俊伟 1陆桂丽 1汪萍 1王杰 1魏玉荣 1魏婕 1米晓云 1沙依兰·卡依扎1
作者信息
- 1. 新疆畜牧科学院兽医研究所(新疆畜牧科学院动物临床医学研究中心)/新疆动物疫病研究重点实验室,乌鲁木齐 830013
- 折叠
摘要
[目的]研究新疆某规模化牛场犊牛腹泻症的主要病原.[方法]采用RT-PCR方法,检测14 份腹泻犊牛粪便中的病毒,对4 头死亡犊牛肝组织进行细菌分离培养及鉴定、小鼠致病性和药物敏感性试验.将分离的致病菌扩增16S rDNA基因片段并测序,并将其结果在NCBI中用BLAST搜索同源序列,分析遗传进化特性.[结果]该牛场犊牛腹泻的病原为牛冠状病毒(Bovine coronavirus,BCoV)、牛轮状病毒(Bovine rotavirus,BRV)、大肠埃希氏杆菌(Escherichia coli,E.coli)和肺炎克雷伯氏菌(Klebsiella pneumoniae,K.pneumoniae).14 份粪便中,BCoV检出率为 64.29%,BRV检出率为 50.00%;4 份肝组织中,E.coli检出率为 100.00%,K.pneumoniae检出率为50.00%.分离的2 种致病菌E.coli和K.pneumoniae对阿米卡星敏感,对其它16 种抗生素均耐药.[结论]该牛场引起犊牛腹泻的主要病原为BCoV、BRV、E.coli和K.pneumoniae,存在病毒与细菌的混合感染.选择阿米卡星药物防治E.coli和K.pneumoniae.
Abstract
[Objective]This article aims to identify the main pathogens of calf diarrhea in a large-scale cattle farm in Xinjiang.[Methods]Virus detection was performed on 14 diarrhea calf feces using RT-PCR.Besides,bacteria were isolated from the liver tissues of four dead calves,cultured and identified,and mouse pathogenicity and drug sensitivity tests were conducted on these bacteria.Afterwards,the 16 S rDNA gene fragment of the isolated pathogenic bacteria was amplified and sequenced,and the sequencing results were searched for homologous sequences using BLAST in NCBI for genetic evolution analysis.[Results]The patho-gens of diarrhea in the cattle farm were Bovine coronavirus(BCoV),Bovine rotavirus(BRV),Escherichia co-li(E.coli),and Klebsiella pneumoniae(K.pneumoniae).Among the 14 feces,the detection rate of BCoV was 64.29%,and the detection rate of BRV was 50.00%;Among the four liver tissues,the detection rate of E.coli was 100.00%,and the detection rate of K.pneumoniae was 50.00%.The two isolated pathogenic bacteria E.coli and K.pneumoniae were sensitive to amikacin and resistant to all 16 other antibiotics.[Con-clusion]The main pathogens causing calf diarrhea in this cattle farm are BCoV,BRV,E.coli,and K.pneu-moniae,with mixed infection of viruses and bacteria.Amikacin can be used as the preferred drug for the pre-vention and treatment of E.coli and K.pneumoniae.
关键词
犊牛腹泻/病原学/检测/鉴定Key words
calf diarrhea/etiology/detection/identification引用本文复制引用
基金项目
新疆维吾尔自治区自然科学基金(2021D01A60)
新疆维吾尔自治区奶产业技术体系建设项目(XJARS-11)
出版年
2024
国家科技期刊平台
NSTL
万方数据