Analysis of genetic effects of candidate genes for lambing numbers in different sheep breeds
[Objective]To explore candidate genes affecting lambing number in different sheep breeds.[Methods]Four sheep breeds(468 multi-wave sheep,152 German merino sheep,157 Suffolk sheep and 600 lake sheep)were used as samples,and the genetic polymorphisms of four fecundity candidate genes COIL,FSHR,GUCY1A1 and BMPRIB were detected by amplification hindered mutation system PCR technolo-gy(ARMS-PCR)and restriction enzyme fragment length polymorphism(RFLP),and the correlation be-tween polymorphisms and lambing number was analyzed.[Results]The frequency of homozygous GG genotype in C-site of coil gene g.7321466G>C was about 0.3 in Duolang,German Merino,Suffolk and Hu sheep,CC genotype was significantly higher than that of CG and GG,and CG genotype was significantly higher than that of GG.The GG genotype in German merino sheep was significantly higher than that of CG and CC,and the CG genotype was significantly higher than that of GG.In Suffolk and Hu sheep,the GG and CG genotypes were significantly higher than those of CC.The association between BMPRIB gene genotype and lambing number in Hu sheep showed that the number of lambing of BB and B+genotypes was significantly higher than that of++,but the effect of other breeds was not satisfactory.The T locus of FSHR gene g.75320741C>and the g.43266624G>A locus of CUCY1A1 gene had low effects on lambing in sheep.[Conclusion]The g.7321466G>C locus of COIL gene is expected to replace BMPRIB gene as a molecular marker for the breeding of lamb number traits.
国家科技期刊平台
NSTL
万方数据
