Variation analysis of sweetness gene Sugary1(Su1)sequence in Sweet corn
[Objective]The Sugary1(Su1)gene of sweet corn was analyzed by nucleotide polymorphism analysis and neutral test,and the allelic variation sites of Su1 in sweet corn from different sources were ana-lyzed,which provided a theoretical basis for variety improvement and quality improvement of sweet corn.[Methods]In order to illustrate the sequence polymorphism of maize gene Su1,the Su1 gene in 170 sweet corn inbred lines was sequenced,using the genomic sequence of B73 as the reference.[Results]Analysis showed that 97 nucleotide sites were mutated,including 85 SNPs sites and 12 InDels locus.Among them,there were 12 SNPs loci and 0 InDel in the coding region,85 SNPs loci and 12 InDels in the non-coding re-gion.There were 5 synonymous mutations at SNP site and 7 non-synonymous mutations in the coding region.The inbred lines were divided into 76 haplotypes according to the full-length sequence of Su1 gene,and 47 haplotypes were divided into 47 haplotypes according to the coding region of the gene.[Conclusion]The Su1 gene had undergone obvious artificial selection.
万方数据
维普
