新疆农业科学2024,Vol.61Issue(7) :1605-1614.DOI:10.6048/j.issn.1001-4330.2024.07.006

甜玉米基因Sugary1(Su1)序列的变异分析

Variation analysis of sweetness gene Sugary1(Su1)sequence in Sweet corn

杨彩霞 顾炜 关媛 瞿静涛 党冬冬 吴鹏昊 郑洪建
新疆农业科学2024,Vol.61Issue(7) :1605-1614.DOI:10.6048/j.issn.1001-4330.2024.07.006

甜玉米基因Sugary1(Su1)序列的变异分析

Variation analysis of sweetness gene Sugary1(Su1)sequence in Sweet corn

杨彩霞 1顾炜 2关媛 2瞿静涛 2党冬冬 2吴鹏昊 3郑洪建2
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作者信息

  • 1. 新疆农业大学农学院,乌鲁木齐 830052;上海市农业科学院作物育种栽培研究所/CIMMYT-中国特用玉米研究中心,上海 201403
  • 2. 上海市农业科学院作物育种栽培研究所/CIMMYT-中国特用玉米研究中心,上海 201403;上海特用玉米工程技术研究中心,上海 201403
  • 3. 新疆农业大学农学院,乌鲁木齐 830052
  • 折叠

摘要

[目的]研究甜玉米基因Sugary1(Su1)序列,分析核苷酸多态性及检验,研究不同来源甜玉米中的Su1的等位变异位点,为甜玉米的品种改良和提高品质提供理论基础.[方法]以B73的Su1基因组序列为模板,对170份甜玉米自交系的Su1基因进行测序,研究玉米Su1基因序列的多态性.[结果]共有97个核苷酸位点发生变异,包含85个SNP位点,12个InDel位点.其中,位于编码区的SNP位点12个,InDel位点0个;位于非编码区的SNP位点85个,InDel位点12个.编码区中SNP位点同义突变有5个,非同义突变7个.自交系材料分为76个单倍型,该基因编码区序列将自交系材料分为47种单倍型.[结论]甜玉米Su1基因经历了明显的人工选择.

Abstract

[Objective]The Sugary1(Su1)gene of sweet corn was analyzed by nucleotide polymorphism analysis and neutral test,and the allelic variation sites of Su1 in sweet corn from different sources were ana-lyzed,which provided a theoretical basis for variety improvement and quality improvement of sweet corn.[Methods]In order to illustrate the sequence polymorphism of maize gene Su1,the Su1 gene in 170 sweet corn inbred lines was sequenced,using the genomic sequence of B73 as the reference.[Results]Analysis showed that 97 nucleotide sites were mutated,including 85 SNPs sites and 12 InDels locus.Among them,there were 12 SNPs loci and 0 InDel in the coding region,85 SNPs loci and 12 InDels in the non-coding re-gion.There were 5 synonymous mutations at SNP site and 7 non-synonymous mutations in the coding region.The inbred lines were divided into 76 haplotypes according to the full-length sequence of Su1 gene,and 47 haplotypes were divided into 47 haplotypes according to the coding region of the gene.[Conclusion]The Su1 gene had undergone obvious artificial selection.

关键词

甜玉米/Sugary1/等位变异/中性检验/单倍型

Key words

sweet corn/Sugary1/allelic variation/neutral test/haplotype

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基金项目

上海市科学技术委员会科技计划项目(20392000400)

上海市科技兴农项目(2022-02-08-00-F01190)

出版年

2024
新疆农业科学
新疆农业科学院 新疆农业大学 新疆农学会

新疆农业科学

CSTPCD北大核心
影响因子:0.698
ISSN:1001-4330
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