Identification of genome size and chromosomal ploidy of three prunus species by flow cytometry
[Objective]To explore FCM detection system suitable for different Prunus resources,estimate their genome size and ploidy,and provide basis for the identification,classification,genome and transcriptome of Prunus resources.[Methods]Three commonly used nuclear lysates were screened and optimized by FCM,and the most suitable lysates were selected to detect the genome size and chromosome ploidy of three plum plants.[Results]LB01,Marie's and WPB lysates were all effective in the detection of P.simonii Carr.,and WPB lysates was the best.The two lysates Marie's and LB01 did not match with P.domestica L.,and the im-proved WPB lysate was the most suitable for P.simonii Carr.and LBO1 lysate did not match,WPB lysate had better cracking effect on P.simonii Carr.than Marie's lysate,and the improved WPB lysate had the best crack-ing effect on P.simonii Carr.The two lysates Marie's and LBO1 did not match with P.domestica L.,and the improved WPB lysate was the most suitable for P.domestica L..The P.simonii Carr.and LB01 lysate did not match,WPB lysate had better cracking effect on P.simonii Carr..than Marie's lysate,and the improved WPB lysate had the best cracking effect on P.simonii Carr.With genome sizes ranging from 491.22 to 566.40 Mb and 514.46 to 573.83 Mb,respectively,with an average genome size of 1,678.37 Mb.[Conclusion]The i-dentified germplasm resources of P.cerasifera Ehrh.and P.simonii Carr.were all diploid,while the P.domestica L.germplasm resources were all hexaploid,the genome size of different varieties in each species of Prunus genus is different.
万方数据
维普
