Effects of increased application of microbial fertilizer on soil microbial diversity of cucumber in facilities
[Objective]In order to study the effect of increased application microbial fertilizer on the structure and function of soil microbial community.The results of this study can provide a theoretical basis for the use of microbial fertilizer,ecological management,soil improvement and increasing vegetable yield in the process of protected vegetable cultivation.[Methods]Cucumber was taken as the experimental material,soil microorganisms as the research object,and soil without microbial fertilizer as the control,and 16SrRNA and fungal ITS region sequencing were used to study the effect of microbial fertilizer on the structure and function of soil community.[Results]Increasing microbial fertilizer application could increase the bacterial abundance of Bacteroidetes,Chloroflexium and Firmicutes,and the fungal abundance of basidiomycetes,and decrease the fungal abundance of Ascomycetes and Perispora.It could also increase the uniformity of microorganisms in soil,the bacterial diversity and reduce the diversity of fungi.By increasing the content of beneficial bacteria and reducing pathogenic fungi,the population could be enriched and the function of soil microbial community effectively improved,thus causing metabolic function of microbial community stronger than that of control soil.[Conclusion]The application of microbial fertilizer can improve the soil nutrient environment,provide suffi-cient nutrients and energy for the life activities of soil microorganisms,thereby promoting the growth and repro-duction of microorganisms,enriching the population,and effectively improving the function of soil microbial communities.
万方数据
维普
