Structure prediction of the ACC protein from Pontibacter kalidii XAAS-72T with the plant growth-promoting character
[Objective]To explore the plant growth promotion function of a novel strain Pontibacter kalidii XAAS-72T,and investigate the potential functional genes.[Methods]The strain whole genome was se-quenced,and the composition of function genes were analyzed.A candidate gene related to ACC deaminase synthesis was obtained,and the enzyme protein characteristics were predicted.[Results]The results showed that the growth of wheat seed treated with Pontibacter kalidii XAAS-72T was promoted significantly in pots.The genome length of strain XAAS-72(accession no.CP1 11079)was 5,054,860 bp,containing one circular plasmid.A total GC content was 54.52％,and the number of annotated genes was 4,391,coding for 4,261 proteins.A variety of resistance and growth-promoting related genes was observed.It had the highest similarity of 72.48％to the ACC deaminase of Pontibacter sp.BAB1700.It an unstable hydrophilic protein,with none of transmembrane structure and signal peptide structure.[Conclusion]Pontibacter kalidii XAAS-72T harbors a lot of genes related to the stress resistance and the plant growth-promoting.
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