Effects of different types of salt and alkali stress on cotton seed germination
[Objective]This study aims to investigate the influence and threshold of different types of sa-line-alkali stress on the germination of cotton seeds.[Methods]Four types of saline-alkali stress and sev-en concentration levels were simulated by Petri dish method.Cotton seeds of Xinluzhong 84,Tahe 2,Xin-luzhong 40 and Jiumian 18 were used as research objects.The germination potential,germination rate,germi-nation index,seed vigor index,fresh weight and dry weight of seedlings after germination were analyzed,salt tolerance threshold and membership function values were calculated,and comprehensive evaluation was carried out.[Results](1)The germination potential,germination rate,germination index,vitality index,fresh weight and dry weight of cotton seed after germination showed a significant downward trend under the four types of saline-alkali stress.There was a significant negative correlation between saline-alkali concentration and all indexes,and the correlation between saline-alkali concentration and germination rate was more than-0.90.(2)The salt tolerance thresholds of cotton at germination stage were different under the four types of saline-alkali stress conditions.The salt tolerance thresholds under S1 stress ranged from 90.70 to 121.73 mmol/L.The salt tolerance threshold under S2 stress ranged from 82.68 to 128.30 mmol/L.The salt tolerance threshold under S3 stress ranged from 51.97 to 84.62 mmol/L.The salt tolerance threshold under S4 stress ranged from 73.49 to 98.21 mmol/L.(3)The salt tolerance of cotton seeds under four kinds of saline-alkali stress was analyzed by membership function and its order was Xinluzhong 84>Xinluzhong 40>Tahe 2>Jiumian 18 under S1 stress,Xinluzhong 84>Tahe 2>Xinluzhong 40>Jiumian 18 under S2 stress,and Tahe 2>Xinluzhong 40>Xinluzhong 84>Jiumian 18 under S3 stress.It was found that the order of salt tolerance of cotton seeds under S4 stress was consistent with that under S2 stress.[Conclusion]The germina-tion potential,germination rate,germination index and vigor index of cotton seeds are decreased under the four types of saline-alkali stress,and the fresh and dry weight of seedlings shows a downward trend with the in-crease of the concentration.The effect of alkaline salt on the germination of cotton seed is obviously greater than that of neutral salt.