Quantity and Distribution Characteristics of Functional Microorganisms in Gastrointestinal Tract of Liuyang Black Goats
The aim of this study was to explore the quantity and distribution characteristics of functional microorganisms in gastrointestinal tract of Liuyang Black goats,and provide foundation for further study on nutritional metabolism of these microbes.Microbes with known definite functions,such as bacteria and methogen,four cellulolytic bacteria,probiotics (Lactobacillus and Bifidobacterium),Prevotella ruminicola were selected in this study.Four Liuyang Black goats (1.5-year-old,(20.0±1.2)kg weight) were slaughtered at the same time and the digesta in the duodenum,jejunum,ileum,cecum,colon,rectum and rumen were collected for the isolation of the DNA,followed by the construction of the recombined standard plasmids.qRT-PCR was used for the absolute quantification of the microbes.The standard plasmids and curves of the microbes and methogen were constructed successfully and could be applied to the research.There were significant differences (P＜0.01) in microbes between the 7 sections of the gastrointestinal tract of Liuyang Black goats.And the numbers of the microbes in the same section differed from each other significantly.Apart from the Butyrivibrio fibrisolvens,the largest number of the microbes was found in the rumen.In the intestinal tract,the number of microbes in the duodenum and jejunum was the least,while the number of microbes in the cecum,colon and rectum was the largest.The quantity and distribution characteristics of functional microorganisms in different gastrointestinal tracts of Liuyang Black goats significantly differ from each other (P＜0.01).Overall,the number in the rumen was the biggest; the number in the cecum,colon and rectum was middle,followed by the ileum,while the least number was found in the duodenum and jejunum.This difference in the quantity and distribution characteristics of functional microorganisms in gastrointestinal tract has paved a way for the further study on different nutritional metabolism of these microbes.
gastrointestinal tractqRT-PCRmicroorganismLiuyang Black goat
国家科技期刊平台
NSTL
万方数据
维普
