Transcriptome Analysis of Intramuscular Preadipocytes and Matureadipocyte in Cashmere Goats
The purpose of this study was to discover differentially expressed genes between before and after the intramuscular fat cell maturation of Cashmere goats,and find out major signal transduction pathways.Then,the genes associated with fat deposition were screened.The highthroughput sequencing for transcriptome before and after intramuscular fat cell maturation in Cashmere goats was performed by using MiSeq (Illumina).Other bioinformatics methods were also taken,such as sequencing assess,gene function annotation and alternative splicing of the genes identified.A total of 93 930 contigs were obtained by assembling.The value of N50 was 1 586 nt.The 78 differential expression genes are found (Fold Change＞ 4,FDR＜ 0.01)by CLCGenomicsworkbench6 software.According to the GO function annotation,these genes could be divided into 46 branches,including skeletal muscle development,extracellular region,insulinlike growth factor binding,et al.According to the KEGG database,the differential genes were mainly involved in these pathways,such as the phagosome,type Ⅰ diabetes and rheumatoid arthritis,et al.The four mainly splicing types in this study were A3SS(Alternative 3'splice site),A5SS (Alternative 5'splice site),SE(Exon skipping)and RI((Intron retention).In fact,A3SS was obvious in the intramuscular adipocytes of goat.The high-throughput RNA-seq provided the whole transcript information,the important functional genes were obtained in the process of proliferation and differentiation for intramuscular fat cell.The up-regulation genes might play important roles in the process of insulin-like growth factor binding and diabetes mellitus,which will lay a foundation for further studying functional genomics of Cashmere goat fat deposition and improving the quality of goat.
国家科技期刊平台
NSTL
万方数据
维普
