首页|秦川牛CD36基因多态性检测及其与肉用性状的相关性研究

秦川牛CD36基因多态性检测及其与肉用性状的相关性研究

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
旨在研究秦川牛脂肪酸转位酶基因(Cluster of differentiation 36,CD36)的多态性及其对秦川牛肉用性状的影响,以探索改善秦川牛肉用性状的分子育种方法.本研究选取288头同等饲养条件下的24月龄健康秦川牛母牛,采用DNA测序技术检测了CD36基因的多态性,并结合PCR-RFLP技术对所发现的多态位点进行基因型分型,然后将不同基因型与秦川牛肉用性状(背膘厚、眼肌面积、眼肌深度和肌内脂肪含量)进行关联性分析.结果表明,在CD36基因上发现4个多态位点,分别命名为SV1、SV2、SV3和SV4.连锁不平衡和单倍型分析表明,SV1-SV2为连锁位点,与SV3为强连锁,SV4与其他3个位点均为弱连锁,单倍型ITA属于优势单倍型(频率为60.4％).关联分析结果表明,除SV4在眼肌深度指标中存在显著性差异外,单个多态位点的基因型之间和不同多态位点的组合基因型之间均在肌内脂肪含量指标中存在显著性或极显著性差异.其中,IITTAG型个体在肌内脂肪含量指标中显著低于IWTCAA型个体(P＜0.05),极显著低于WWCCAA型个体(P＜0.01).结果提示,CD36基因对秦川牛肉用性状有显著的影响,可通过人工选择降低群体中IITTAG型个体的比例以改善肉用性状.
Association of CD36 Gene Polymorphism with Meat Traits in Qinchuan Cattle
The aim of the study was to research polymorphism of fatty acid translocase gene (Cluster of differentiation 36,CD36) and analyze its effect on meat traits,then find molecular breeding methods of improving meat traits in Qinchuan cattle.In this study,288 24-month-old Qinchuan heifers under similar feeding conditions were randomly selected.Direct DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods were used to genotype the polymorphisms of CD36 gene.The polymorphisms of bovine CD36 gene and its associations with meat traits (back fat thickness,eye muscle area,eye muscle depth and intramuscular fatty content) were analyzed.Four mutation sites were detected,named SV1,SV2,SV3,and SV4,respectively.Further,the linkage disequilibrium and haplotypes were analyzed among the four loci,suggested that SV1 and SV2 were complete linkage,SV1-SV2 and SV3 were strong linked,and SV4 was little linked with SV1-SV2 and SV3,respectively.Haplotype of ITA was dominant (with frequency of 60.4％).Except differentiation of eye muscle depth at SV4,the result of association analysis showed significant or extremely significant difference be tween different genotypes of single and combined sites in intramuscular fatty content in Qinchuan cattle,and the individuals with combined genotype IITTAG had a lower intramuscular fatty content than that of the individuals with combined genotype IITTAG (P＜0.05) and combined genotype WWCCAA (P＜0.01).As the result showed,CD36 gene had a significant effect on meat traits in Qinchuan cattle,and reducing the ratio of individuals with combined genotype IITTAG may be a method to improve meat traits.

Qinchuan cattleCD36PCR-RFLPpolymorphismhaplotypemeat traits

张松、昝林森、王洪宝、王国庆、付常振、张亚冉、梅楚刚、邓冠群

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西北农林科技大学动物科技学院,杨凌712100

国家肉牛改良中心,杨凌712100

秦川牛 CD36 PCR-RFLP 多态性 单倍型 肉用性状

国家863计划国家863计划国家科技支撑计划国家自然科学基金国家转基因育种专项国家现代农业产业技术体系陕西省科技统筹创新工程计划

2013AA1025052011AA100307-022011BAD2804-03312724112011ZX08007-002CARS-382014KTZB02-02

2014

10.11843/j.issn.0366-6964.2014.07.005

畜牧兽医学报
中国畜牧兽医学会

畜牧兽医学报

CSTPCDCSCD北大核心
影响因子：0.729
ISSN：0366-6964
年,卷(期)：2014.45(7)
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