Effects of Resistance Selection in Coordination with Vaccination on MDV-1 Loads in Feather Follicle of Chickens
The aim of the present study was to study the effects of HVT-vaccinated and MD resistant selection on virus loads in feather follicle of chickens after the challenge with MDV-1.Real-time FQ-PCR was used for absolute quantification of the DNA levels of meq of feather follicle sampled in 5 different times(7,14,21,28,35 DPI) from all chickens after the challenge for quantification of MDV-1.The load of MDV-1 was detected in feather follicle to analyzed and compared proliferation of MDV-1 in resistant/common or/and HVT-vaccinated/HVT-unvaccinated chickens.The results showed that 1) from the impact of MD-resistant on the MDV-1 loads,the load of MDV-1 were significantly higher in chickens of F group(common line,HVT-unvaccinated and MDV-challenged) whereas significantly lower(P＜0.05) in chickens of T group(MD-resistant line,HVT-unvaccinated and MDV-challenged) in 14,21 DPI;On the one hand,R group(MD-resistant line,HVT-vaccinated and MDV-challenged) and G group(common line,HVT-vaccinated and MDV-challenged) give about the same load of MDV-1;2) From the impact of HVT-vaccinated on the MDV-1 loads,the load of MDV-1 were significantly higher in chickens of T group(MD-resistant line,HVT-unvaccinated and MDV-challenged) whereas significantly lower(P＜0.05) in chickens of R group(MD-resistant line,HVT-vaccinated and MDV-challenged) in 28 DPI;On the one hand,the load of MDV-1 were significantly higher in chickens of F group(common line,HVT-unvaccinated and MDV-challenged) whereas significantly lower(P＜0.05)in chickens of G group (common line,HVT-vaccinated and MDV-challenged) in 14,21 DPI.3) From the impact of MD-resistant and HVT-vaccinated on the MDV-1 loads,the load of MDV-1 were significantly higher in chickens of F group(common line,HVT-unvaccinated and MDV-challenged) whereas significantly lower in chickens of R group(MD-resistant line,HVT-vaccinated and MDV-challenged) in 14(P＜0.05),28(P＜0.01) and 35(P＜0.05)DPI.These results indicated that the virus loads in feather follicle of chickens can be reduced significantly by resistance selection in coordination with vaccination,which will reduce the risk of MDV-1 transmission and improve the chicken's chance of survival.
国家科技期刊平台
NSTL
万方数据
维普
