旨在对德保矮马(Debao pony)X染色体的选择信号进行筛选.本研究利用Illumina公司开发的马芯片Equine 65K SNP BeadChip,对德保矮马、伊犁马、蒙古马进行X染色体扫描,获得2 339个SNPs位点.通过群体分化系数FsT法和XP-EHH两种不同的方法,分别以伊犁马和蒙古马为对照群体对德保矮马进行X染色体选择信号检测.结果,筛选到两个受到较强选择区域4.0～39.9和87.1～123.5 Mb,包含64个“离群位点”.通过基因注释筛选到PHEX、BCOR、PNPLA4和GPC3等与生长和骨骼发育相关的基因.研究结果发现,德保矮马的选育过程中X染色体很多与生长相关的基因受到了强烈的选择,其中部分在马中未见报道,可以作为研究矮小性状的重要候选基因.
Detecting Selection Signatures on X Chromosome of Debao Pony
The study aimed to screen the selection signatures on X chromosome of Debao pony.The Illumina Equine 65K SNP BeadChip was used to perform a X-chromosomal scan on Debao pony,Yili horses and Mongolian horses and obtain 2 339 SNPs with high quality.The result showed that,based on these SNPs,we detected 2 regions(4.0-39.9 and 87.1-123.5 Mb) under strong selection on the X chromosome of Debao pony by 2 different methods,FST and XP-EHH.The 2 detected regions contained 64 outliers overlapping genes including BCOR,PHEX,PNPLA4 and GPC3,which are related to the body size and the bone development.The results indicate that genes associated with growth on X chromosome are subject to strong artificial selection pressure during the breeding of the smallest Chinese pony.A few of them are not reported before and could be important candidate genes in future study.
国家科技期刊平台
NSTL
万方数据
维普
