Association of CRTC3 Gene Polymorphisms and Genotype Combination with Growth Traits of Qinchuan Cattle
The aim of this study was to investigate the possibility of CRTC3 gene as candidate gene for growth performance in Qinchuan cattle,and search the molecular markers related to Qinchuan cattle growth traits.PCR-RFLP method was applied to detect the polymorphisms of CRTC3 in 395 healthy Qinchuan cattle,and the association of its polymorphisms and genotype combination with growth traits of Qinchuan cattle was also analyzed.The result showed that 2 SNPs were identified,including G66478C in exon and C91297T in intron.At the G66478C locus,the cattle with genotype GC had greater body length and chest depth values than those with genotype CC(P＜0.01 and P＜0.05,respectively).At the C91297T locus,animals with genotype CT had greater body length,hip height,rump length and chest depth values than those with genotype TT(P ＜0.01).Animals with CC-TT had significantly greater hip height and rump length values than those with CC-CT(P ＜ 0.01),and chest depth and chest circumference values for CC-TT were greater than CC-CT(P＜0.05).Therefore,variations in the CRTC3 gene and the genotype combination CC-CT may be useful as molecular markers for growth traits in Qinchuan cattle breeding.
国家科技期刊平台
NSTL
万方数据
维普
