Association Analysis of AQP9 and RPS 10 Gene Polymorphisms with Backfat Thickness in Beijing Black Pigs
This study aimed to explore the gene polymorphisms of aquaporins9(AQP9)and ribosomal protein S10(RPS10)and their correlation with backfat thickness in Beijing black pigs,in order to obtain effective markers of backfat thickness in Beijing black pigs.The backfat thickness of 413 Beijing black pigs was measured at different parts(shoulder,6-7 intercostal,thoracolumbar,lumbosacral).The promoter and exon sequences of AQP9 and RPS10 genes were amplified by PCR to screen SNP sites,and the association between SNPs and backfat thickness was analyzed.Differential gene expression was detected by fluorescence quantitative PCR.The results showed that there were 4 mutation sites in the promoter region of AQP9 gene,among which one SNP,rs332699245 A＞C,was significantly associated with backfat thickness at the thoracolumbar(P＜0.05),and the mutation of this site predicted the change of binding transcription factors.There were 8 mutation sites of RPS10 gene in 3'UTR and CDS region,5 of which were significantly associated with backfat thickness:rs80795904 C＞G and rs80932213 T＞C were significantly correlated with 6-7 intercostal backfat thickness(P＜0.05),rs3469834461 C＞T were significantly correlated with shoulders backfat thickness and 4 regions average backfat thickness(P＜0.05),rs80862457 C＞T was significantly associated with backfat thickness at the thoracolumbar and lumbosacral(P＜0.05),and rs336938272 A＞C was significantly associated with 6-7 intercostal backfat thickness,backfat thickness at lumbosacral and 4 regions average backfat thickness(P＜0.05).One SNP(rs80862457 C＞T)was detected in the 3'UTR region,which was located at the binding target of small RNA(miRNA).Analysis of gene expression differences showed that the AQP9 mRNA expression of AA type individuals in rs332699245 A＞C was significantly higher than that of AC type individuals(P＜0.05),and the RPS10 mRNA expression of TT type individuals in rs80862457 C＞T was significantly higher than that of CC type individuals(P＜0.05).In summary,AQP9 and RPS10 genes were significantly associated with backfat thickness of Beijing black pigs,and rs332699245 A＞C and rs80862457 C＞T sites can be used as potential molecular markers for backfat thickness breeding of Beijing black pigs,providing theoretical support for breeding of Beijing black pigs.
Beijing black pigsAQP9RPS10backfat thicknessassociation analysis
国家科技期刊平台
NSTL
万方数据
维普
