基于全基因组关联分析揭示肉鸡腿病发生的遗传机制
Genetic Mechanism of Broiler Leg Disease Based on Genome-Wide Association Analysis
唐鑫鑫 1郑炬梅 2骆娜 2营凡 3朱丹 3李森 3刘大伟 3安炳星 2文杰 2赵桂苹 2李和刚4
作者信息
- 1. 青岛农业大学动物科技学院,青岛 266109;中国农业科学院北京畜牧兽医研究所,北京 100193
- 2. 中国农业科学院北京畜牧兽医研究所,北京 100193
- 3. 佛山市高明区新广农牧有限公司,佛山 528000
- 4. 青岛农业大学动物科技学院,青岛 266109
- 折叠
摘要
旨在揭示肉鸡腿病发生的遗传机制以降低肉鸡腿病发生率.采用我国自主培育的"广明2号"多品系、多世代共2 331只白羽肉鸡公鸡作为试验素材,于42日龄通过X光鉴定腿部健康状况,利用"京芯一号"55K SNP芯片对全部个体进行基因分型,使用plink运用二分类性状的逻辑回归模型(logistic regression model,LR Model)对腿部健康表型进行全基因组关联分析(genome-wide association study,GW AS).将不同群体芯片数据合并进行质控后,保留2 330个个体(2 256只健康,74只患病)和30 414个SNPs位点用于后续分析.通过GW AS分析筛选到5个潜在SNPs位点,分布在6号和18号染色体上,分别注释到与腿病相关的3个功能候选基因TBCD、SIRT1和PBLD上.本研究为白羽肉鸡腿部健康表型提供了重要的遗传位点和候选基因,为推进肉鸡抗病育种进程提供遗传基础.
Abstract
This study aimed to reveal the genetic mechanism of leg disease in broilers,with the objective of mitigating the incidence of leg disease in broilers.This study employed a comprehensive approach involving 2 331 white-feathered broilers of multi-strain and multi-generation of"Guangming No.2"independently bred in China.The assessment of leg health and diseases was performed by X-ray analysis at 42 days of age.For genotyping,the"Jingxin No.1"55K SNP microarray was used for all individuals,and the genome-wide association study(GWAS)of leg health phenotypes was performed utilizing a logistic regression model tailored for dichotomous traits via plink software.After the integration and meticulous quality control of microarray data across diverse populations,a total of 2 330 individuals(including 2 256 healthy and 74 leg disease subjects)and 30 414 SNPs loci were deemed suitable for subsequent analysis.Through the GW AS analysis,5 potential SNPs loci,distributed on chromosomes 6 and 18,were identified.Additionally,the investigation led to the annotation of 3 functional candidate genes,namely TBCD,SIRT1,and PBLD,which are directly related to leg disease.This study results provide important genetic loci and candidate genes for the healthy leg phenotype of white-feathered broilers,and provides a genetic basis for advancing the breeding process of broiler disease resistance.
关键词
白羽肉鸡/腿病/全基因组关联分析/候选基因Key words
white-feathered broiler/leg disease/genome-wide association analysis/candidate genes引用本文复制引用
基金项目
国家自然科学基金(32230101)
十四五国家重点研发计划课题(2022YFF1000203)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据