Genetic Mechanism of Broiler Leg Disease Based on Genome-Wide Association Analysis
This study aimed to reveal the genetic mechanism of leg disease in broilers,with the objective of mitigating the incidence of leg disease in broilers.This study employed a comprehensive approach involving 2 331 white-feathered broilers of multi-strain and multi-generation of"Guangming No.2"independently bred in China.The assessment of leg health and diseases was performed by X-ray analysis at 42 days of age.For genotyping,the"Jingxin No.1"55K SNP microarray was used for all individuals,and the genome-wide association study(GWAS)of leg health phenotypes was performed utilizing a logistic regression model tailored for dichotomous traits via plink software.After the integration and meticulous quality control of microarray data across diverse populations,a total of 2 330 individuals(including 2 256 healthy and 74 leg disease subjects)and 30 414 SNPs loci were deemed suitable for subsequent analysis.Through the GW AS analysis,5 potential SNPs loci,distributed on chromosomes 6 and 18,were identified.Additionally,the investigation led to the annotation of 3 functional candidate genes,namely TBCD,SIRT1,and PBLD,which are directly related to leg disease.This study results provide important genetic loci and candidate genes for the healthy leg phenotype of white-feathered broilers,and provides a genetic basis for advancing the breeding process of broiler disease resistance.
white-feathered broilerleg diseasegenome-wide association analysiscandidate genes