摘要
牛病毒性腹泻病原严重危害畜牧业生产,BVDV、BoAstV、BCoV、MRV、BKoV、BoNeV、BNoV、BRV、BToV被认为是引起牛群腹泻的病原,不仅如此,BVDV、BCoV和BToV除了能引起消化道症状外还能引起呼吸道症状,BVDV不仅能引起消化道和呼吸道症状,还会导致母牛生殖功能异常,这些疾病对畜牧业造成巨大的经济损失.为了解牛主要病毒性腹泻病原流行情况,本次试验通过PCR检测方法对我国河北地区323份腹泻牛粪样品进行常见9种病毒性牛腹泻病原进行检测.结果显示,BVDV阳性检出率1.85％(6/323)、BCoV阳性检出率14.24％(46/323)、BRV 阳性检出率 57.89％(187/323)、BoAstV 阳性检出率 0.31％(1/323)、BoNeV 阳性检出率10.84％(35/323)、BNoV 阳性检出率 4.33％(14/323)、MRV 阳性检出率 2.48％(8/323)、BToV 阳性检出率4.64％(15/323)、BKoV 阳性检出率 11.76％(38/323).混合感染共有 76 份,占比 23.53％(76/323).HBLF2302株的全基因序列、S、HE、N、M和E基因进行同源性和遗传进化分析发现,HBLF2302与BCoV/NMG1/2022基因同源性最高,为GⅡb亚型.基于氨基酸序列比对和HBLF2302的S蛋白三级结构预测发现,在S蛋白中157号位突变为157T,854号位点突变为854I.318V为独特突变,改变了与侧链残基的作用力,与631C形成氢键连接.并发现中国株区别于其它地区的GⅡb亚型,位于ORF1a区域有三个独特位点.本研究丰富了牛腹泻病原的流行病学数据,为牛腹泻病的防控奠定基础.
Abstract
Cattle viral diarrhea pathogens have adverse effects on animal husbandry.BVDV,BoAstV,BCoV,MRV,BKoV,BoNeV,BNoV,BRV,BToV were well known as the cause of diarrhea.On the other hand,BVDV,BCoV and BToV also caused respiratory system symp-toms.Among them,BVDV not only causes alimentary tract symptoms and respiratory symp-toms,but also leads to abnormal reproductive function of cows causing great economic losses.In order to understand the prevalence of the main viral diarrhea pathogens in cattle,323 fecal sam-ples from diarrheal cow in Hebei province of China were detected by PCR to detect nine common viral diarrhea pathogens.Prevalence of 1.85％(6/323),14.24％(46/323).57.89％(187/323),0.31(1/323),10.84％(35/323),4.33％(14/323),2.48％(8/323),4.64％(15/323),11.76％(38/323),was determined for BVDV,BCoV,BRV,BoAstV,BoNeV,BNoV,MRV,BToV,BKoV,respectively.There were 76 co-infection samples accounting for 23.53％(76/323).Homology and genetic evolution analysis of the complete genomic sequence,S,HE,N,M and E genes of HBLF2302 strain showed that HBLF2302 gene had high homology with BCoV/NMG1/2022 and was GⅡb subtype.Based on the amino acid sequence alignment and the tertiary structure prediction of HBLF2302 S protein,it was found that the 157 site was mutated to 157T and the 854 site was mutated to 854I in the S protein.318V is a unique mutation,which changes the force with the side chain residue and forms a hydrogen bond connection with 631C.It was found that the Chinese strain was different from the GⅡb subtype in other regions,and there were three unique sites in the ORF1a region.This study enriched the epidemiological data about bovine diarrhea pathogens for laying the foundation of bovine pathogens prevention and control.
基金项目
"十四五"国家重点研发计划(2021YFD1801101)
陕西省重点研发计划一般项目(2022NY-098)
贵州省种业发展项目()()
江苏省农业科技自主创新资金项目(CX213137)
维普
万方数据