Detection of Bovine Viral Diarrhea Pathogens and Evolution Analysis of Bovine Coronavirus in Langfang,Hebei
Cattle viral diarrhea pathogens have adverse effects on animal husbandry.BVDV,BoAstV,BCoV,MRV,BKoV,BoNeV,BNoV,BRV,BToV were well known as the cause of diarrhea.On the other hand,BVDV,BCoV and BToV also caused respiratory system symp-toms.Among them,BVDV not only causes alimentary tract symptoms and respiratory symp-toms,but also leads to abnormal reproductive function of cows causing great economic losses.In order to understand the prevalence of the main viral diarrhea pathogens in cattle,323 fecal sam-ples from diarrheal cow in Hebei province of China were detected by PCR to detect nine common viral diarrhea pathogens.Prevalence of 1.85％(6/323),14.24％(46/323).57.89％(187/323),0.31(1/323),10.84％(35/323),4.33％(14/323),2.48％(8/323),4.64％(15/323),11.76％(38/323),was determined for BVDV,BCoV,BRV,BoAstV,BoNeV,BNoV,MRV,BToV,BKoV,respectively.There were 76 co-infection samples accounting for 23.53％(76/323).Homology and genetic evolution analysis of the complete genomic sequence,S,HE,N,M and E genes of HBLF2302 strain showed that HBLF2302 gene had high homology with BCoV/NMG1/2022 and was GⅡb subtype.Based on the amino acid sequence alignment and the tertiary structure prediction of HBLF2302 S protein,it was found that the 157 site was mutated to 157T and the 854 site was mutated to 854I in the S protein.318V is a unique mutation,which changes the force with the side chain residue and forms a hydrogen bond connection with 631C.It was found that the Chinese strain was different from the GⅡb subtype in other regions,and there were three unique sites in the ORF1a region.This study enriched the epidemiological data about bovine diarrhea pathogens for laying the foundation of bovine pathogens prevention and control.
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