The aim was to investigate the regulatory effect of a monoclonal antibody against D1133L protein of African swine fever virus(ASFV)during virus replication.Based on the preparation of monoclonal antibody against D1133L protein,ASFV and different concentrations of monoclonal antibodies were added simultaneously to PAMs,and the effect of monoclonal antibody against D1133L on virus replication was analyzed by 50％Hemadsorbing dose(HAD5o),real-time quantitative PCR(RT-qPCR),Western blot,and observation of fluorescence.Monoclonal antibodies at different concentrations significantly inhibited the viral titer(P＜0.01),the expression level of proteins,the gene transcription level(P＜0.01),and the expression level of ASFV-GFP green fluorescent protein(P＜0.05)at different infection times in a dose-dependent manner.In summary,monoclonal antibody against D1133L significantly inhibited ASFV replication,the results of the trial are informative in the selection of drug targets for ASFV.
关键词
非洲猪瘟病毒/D1133/L蛋白/单克隆抗体
Key words
African swine fever virus/D1133 L protein/monoclonal antibody
万方数据