Proteomic Analysis of Sperm with Different Freezability in Chinese Holstein Bulls
In dairy cattle breeding systems dominated by cryopreserved semen and artificial insemination,the post-freezing motility of bull sperm is of great interest.Sperm freezability is the key to the post-freezing sperm motility.Previous studies have shown significant differences in sperm freezability among different bulls.Therefore,it is necessary to explore genes and molecular markers that affect sperm freezing tolerance at the genetic level,in order to lay a foundation for improving the sperm freezability of Chinese bulls.The present study investigated the sperm proteome of Holstein bulls in the high sperm freezability group(n=9)and the low sperm freezability group(n=6).The fresh sperm motility of bulls in the high sperm freezability group was greater than 0.65 and post-freezing motility greater than 0.4,while the fresh sperm motility of bulls in the low sperm freezability group was greater than 0.65 and post-freezing motility less than 0.27.Quantitative proteomic analysis was conducted on sperm cells of 15 bulls using label-free proteomics technology followed by bioinformatics analysis.As a result,a total of 432 differentially expressed proteins were identified between the high and low freezability groups,which were mainly enriched in biological processes closely related to sperm energy supply,such as metabolic pathway and oxidative phosphorylation.The analysis of protein-protein interaction networks showed that there were strong interactions between proteins related to energy metabolism.Compared with the QTL database,it was found that 9 differentially expressed proteins overlapped with genes associated with percentage live sperms after thawing.Overall,several important candidate proteins were identified as potential biomarkers for the freezing resistance of bull sperms,such as HSPA1A,BSP3 and ACSL4.This study revealed the proteomic characteristics of bull sperms with different freezability,which provides important information for molecular breeding of frozen semen quality in Chinese bulls.
万方数据
维普
