In the study,we found that one type of tumors in yellow-feather chicken that look like a white,stiff,irregular shaped mass,growing up at the position between comb and eyelid.In order to identify the pathogenetic characteristics and causative agent of the disease,we have made a series of diagnoses and studies of the disease.Research has found that about 2％sick chicken in the laying hens,they have no other pathological changes other than the tumors.The histopathological diagnosis the pathological tissue was Hemangioendothelioma.RT-PCR tests on tumor tissue showed that the sick chickens were infected with the subgroup K avian leukosis virus(ALV-K).We have tried to isolate the viruses from 32 blood samples,6 tumor tissues and brain tissues of infected chicks.Finally,we successfully isolated 4 strains of ALV-K from brain tissues.The 4 strains of ALV-K were named as GXJL01-GXJL04.To better understand the molecular characterization of GXJLO1-GXJL04.On the other hand,we have isolated another 7 strains of ALV-K from 5 large-scale chicken farms in South China,amplifying and sequencing their env genome.The phylogenetic tree of 11 strains of ALV-K was drawn by MEGA-X software based on gp85 amino acid sequence,indicating that GXJL01-GXJL04 were most similar to the JS15SG01 and fowl glioma-inducing virus(FGV)such as Km5844 and Sp53,and they were distantly related to the genetic evolution of the other 7 ALV-K isolates in this study.What's more,in the analysis of gp85 amino acid point mutation,we have found that some amino acid sites are the same as fowl glioma-inducing virus and JS15SG01,while they are different from other 7 strains of ALV-K from south China.In the study,the tumors of the chicken was firstly diagnosed as Hemangioendothelioma,the associated pathogen was ALV-K,and the isolates GXJL01-GXJL04 were highly homologous to neurotropic strains like FGV,JS15SG01,but were different from the other circulating ALV-K in South China.
维普
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