畜牧兽医学报2024,Vol.55Issue(3) :1208-1216.DOI:10.11843/j.issn.0366-6964.2024.03.032

鹅源副鸡禽杆菌全基因组重测序及比较基因组学分析

Complete Genome Re-sequence and Comparative Genomic Analysis of Avibacterium paragallinarum from Geese

苏文楠 刘佳琪 钟嘉诚 陈济铛 朱婉君 张溢珊 张济培
畜牧兽医学报2024,Vol.55Issue(3) :1208-1216.DOI:10.11843/j.issn.0366-6964.2024.03.032

鹅源副鸡禽杆菌全基因组重测序及比较基因组学分析

Complete Genome Re-sequence and Comparative Genomic Analysis of Avibacterium paragallinarum from Geese

苏文楠 1刘佳琪 1钟嘉诚 1陈济铛 1朱婉君 2张溢珊 2张济培3
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作者信息

  • 1. 佛山科学技术学院,佛山 528225
  • 2. 佛山天牧生物科技有限公司,佛山 528225
  • 3. 佛山科学技术学院,佛山 528225;佛山天牧生物科技有限公司,佛山 528225
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摘要

为了分析鹅源副鸡禽杆菌(Apg)与鸡源菌株的基因组差异,对3株鹅源Apg和4株鸡源Apg进行全基因重测序及基因组分析.全基因重测序结果显示7株菌株基因组片段大小为2.567 832~2.607 127 Mb,GC含量介于40.80%~40.93%之间.SNPs同源性分析结果显示,3株鹅源菌株和鸡源C-AP3株聚集在p4chr1株的小分支上,另外3株鸡源菌株分布在其它国内菌株分支上.基因同源性分析结果显示鹅源菌株特有基因76个,鸡源菌株特有基因37个.对鹅适应性基因的筛查共聚类出79个基因,其中编码已知蛋白的基因有22个,其余编码假定蛋白;对鸡适应性基因的筛查聚类出39个基因,其中10个编码已知蛋白,29个编码假定基因.通过比较基因组学发现与Apg宿主适应性有关的基因为118个,为进一步阐释Apg跨物种感染的分子生物学机制奠定了基础.

Abstract

The aim of this paper was to analyze the differences between the genome of Avibacterium paragallinella(Apg)of goose origin and chicken.In this study,three goose Apg and four chicken Apg isolates were sequenced and their genomic components were analyzed.The results of whole gene re-sequence showed that the genome fragment size of 7 isolates was 2.567 832-2.607 127 Mb,and the GC content was between 40.80%and 40.93%.The results of SNPs homology analysis showed that three strains isolated from geese and three strains of C-AP3 from chicken were clustered on the small branch of p4chr1 strain,and the other three strains from chicken were distributed on the branches of other domestic strains.In the gene homology analysis experiment,there are 76 specific genes of goose isolate and 37 specific genes of chicken isolate.The screening results of adaptive genes in geese showed that 79 genes were co-clustered,including 22 genes encoding known proteins,and the rest encoding putative proteins.In the screening test of chicken adaptive genes,39 genes were clustered,including 10 encoding known proteins and 29 encoding putative genes.Through comparative genomics,found 118 genes related to Apg host adaptation,which laid a foundation for further elucidation of the molecular biological mechanism of Apg cross-species infection.

关键词

副鸡禽杆菌/比较基因组学/全基因重测序/跨物种传播

Key words

Avibacterium paragallinarum/comparative genomics/whole gene resequencing/cross-species transmission

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基金项目

广东省普通高等学校科研项目重点领域专项(2020ZDZX1018)

横向项目(BKH20905901)

出版年

2024
畜牧兽医学报
中国畜牧兽医学会

畜牧兽医学报

CSTPCD北大核心
影响因子:0.729
ISSN:0366-6964
参考文献量21
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