The prime editing system based on CRISPR/Cas9 is a novel gene editing technology,which enables all 12 base-to-base conversions or transition,precise small insertion and deletion without requiring double-strand breaks or exogenous donor DNA templates.This review summa-rizes the development of gene editing approaches,the principle and characteristics of prime edi-ting technique,as well as applications in animal and plant breeding and human gene therapy.It provides a reference for scientists to get insight into prime editing system and further use it in basic research and animal or plant breeding.
关键词
CRISPR/Cas9/引导编辑(prime/editing,PE)/pegRNA/应用研究
Key words
CRISPR/Cas9/prime editing(PE)/pegRNA/application research
维普
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