Sex control is a technique in which a female animal produces offspring with a specific sex through human intervention.Since the separation of X sperm and Y sperm is the technical basis for the realization of sperm sexing in animals,this paper summarizes and compares the new techniques for the separation of sperm before fertilization,including flow cytometry,immunolog-ical approach of sperm sexing,pH separation,microfluidics and nanotechnology.The activation of TLR7/8 signaling pathway by R848(Resiquimod)in immunological isolation is a potential method for sperm isolation,aiming to provide a theoretical basis for sperm sexing techniques.
sperm sexingX spermY spermimmunological approach of sperm sexingTLR7/8