Advances of the Application of Antimicrobial Peptides in the Preservation of Porcine Semen at Room Temperature
Artificial insemination(AI)is one of the core technologies accelerating the development of animal husbandry.Semen preservation is a key part of AI.Porcine semen is susceptible to bac-terial contamination during preservation leading to a decline in sperm quality.With the advent of the era of total antibiotic ban,there is an urgent need to develop antibacterial alternatives with different activities to improve the effect of semen preservation.Antimicrobial peptides(AMPs)have become an important substitute for antibiotics due to their broad-spectrum antimicrobial ac-tivity and lower drug resistance.AMPs play an antibacterial role in the preservation of porcine semen by exerting antibacterial effects through cell membrane damage and non-membrane dam-age mechanisms.This article reviews the research progress of AMPs'antibacterial mechanisms and their application in porcine semen preservation techniques.The aim is to provide a scientific basis for the development of antimicrobial peptide products preserved in porcine semen at room temperature.