Analysis of Microbial Composition Differences in High and Low Feed Conversion Rates Pig Feces based on 16S rRNA Sequencing
Improving feed conversion efficiency in pigs is currently a key focus of pig breeding ef-forts.In this study,FCR values of 384 crossbred sows were ranked,and 20 pigs with extreme feed conversion ratio(10 with the highest FCR and 10 with the lowest FCR)were selected to form the HFCR group and LFCR group,respectively.Fecal samples were collected for 16S rRNA gene sequencing analysis.The results showed that the Richness index,Chao1 index,and ACE index of the LFCR group are significantly higher than those of the HFCR group(P＜0.01).The LEfSe analysis was used to identify microorganisms at the genus level with signifi-cant differences between the two groups.In the LFCR group,microorganisms with LDA＞3 were mainly included Prevotella_1,Treponema_2,Prevotellaceae_NK3B31_group,Eubacteri-um_coprostanoligenes_group,p-1088-a5_gut_group,Prevotella_9,Akkermansia,Prevotel-laceae_UCG_009,Ruminococcaceae_UCG-014,and Ruminococcaceae_NK 4A214_group.In the HFCR group,microorganisms with LDA＞3 were mainly included Megasphaera,Dialister,Catenibacterium,P seudobutyrivibrio,Collin sella,Solobacterium,Subdoligranulum,and Stre ptococcus.The results of Spearman correlation analysis indicated that microorganisms signif-icantly negatively correlated with FCR are mostly associated with short-chain fatty acid produc-tion,while microorganisms significantly positively correlated with FCR were mostly potential pathogenic bacteria.This study screened differential microorganisms in feces from pigs with high and low feed conversion rates,also conducted correlation analysis between the abundance of dif-ferential microorganisms and feed conversion rate traits,and ultimately identified fecal microor-ganisms significantly associated with feed conversion rate,thus provided a reference for the selec-tion of intestinal microbial breeding markers for pig feed conversion rate traits.
万方数据
维普
