The Inhibitory Effect of Salinomycin on Porcine Epidemic Diarrhea Virus in vitro
Porcine epidemic diarrhea(PED)is a porcine intestinal infectious disease caused by por-cine epidemic diarrhea virus(PEDV),characterized by watery diarrhea,vomiting and dehydra-tion.It has caused huge economic losses to the global pig industry,but there are still no effective vaccines and treatments.Salinomycin(SLM)is a commonly used antibiotic with the advantages of low resistance,rapid excretion and very low residue.To explore the inhibitory effect of SLM on PEDV,firstly,the proliferation pattern of PEDV on Vero cells was detected by TCID50,and then the CC50 and IC50 of SLM were measured by CCK-8 test and cytopathic effect(CPE).Final-ly,the co-culture models of SLM,PEDV and Vero cell were established.The effects of SLM on the replication cycle of PEDV were determined by RT-qPCR,Western blot and indirect immuno-fluorescent assay.The results showed that the highest virus titer(107.7·0.1 mL-1)was observed in Ve-ro cells at 24 hours after PEDV infection.The CC50 of SLM on Vero cells was 7.698 µmol·L-1,and the IC50 for PEDV was 1.617 μmol·L-1.The study showed that viral titer,N protein and N gene mRNA expression of PEDV gradually decreased with the increase of SLM concentration.SLM mainly inhibited the replication phase of PEDV,and had no significant effect on the adsorp-tion,invasion and release phases of the virus.The research results could provide new ideas for further exploring PEDV inhibitory drugs.
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