In this study,two strains of Clostridium perfringens type A,named ZWCP209 and ZWCP210,were isolated from the tissues and organs of sudden death Hainan cattle.Genome as-sembly results showed that the genome size was between 3.4-3.5 Mb,and the number of tRNA and CDS was stable.Multilocus sequence analysis(MLST)showed that the sequenced strains be-longed to the same new ST type.The genomes of 27 bovine C.perfringens strains were ob-tained from the NCBI database and analyzed by bioinformatics together with sequenced isolates:A total of 13 resistance genes were detected,among which the tetracycline resistance genes tetA(P)and tetB(P)had the highest carrying rate(79.4％).It is noteworthy that the two Hainan cattle isolates in this study carried more than 7 resistance genes,including optrA,which is resist-ant to oxazolidinone for non-veterinary use.Pan-genome analysis showed that the genomes of these strains contained a total of 7 345 genes,and the number of core genes accounted for 22.94％.In the phylogenetic analysis of the core genome and plc gene,the two Hainan cattle i-solates were in the same branch and had the same virulence factors,with high homology.This study is the first complete genome sequencing and bioinformatics analysis of C.perfringens iso-lated from cattle in Hainan Province in China,which has a reference value for the prevention and treatment of C.perfringens disease in cattle and the study of genome.
关键词
海南黄牛/产气荚膜梭菌/分离鉴定/全基因组测序/生物信息学分析
Key words
Hainan cattle/Clostridium perfringens/isolation and identification/Whole genome sequencing/bioinformatics analysis
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