In order to explore the effect of Toll-interacting protein(Tollip)on the proliferation of Foot-and-mouth disease virus(FMDV),CRISPR/Cas9 gene editing technology was used to con-struct a porcine kidney cell line(PK-15)with Tollip gene deletion,and the knockout effect was confirmed by PCR sequencing and Western blot,Then,CCK-8 kit was used to determine the change of cell viability after Tollip deletion,and there was no obvious difference between knock-out cells and wild cells.Western blot,indirect immunofluorescence,real-time fluorescence and viral titer were used to determine the replication of viruses in knockout cell lines during FMDV infection.The results showed that knockout cells significantly promoted virus replication com-pared with wild cells during FMDV infection.In summary,Tollip deletion cell lines were suc-cessfully constructed on PK-15 cells,and the results showed that Tollip deletion contributed to FMDV replication.These results provide a theoretical basis for the subsequent study of Tollip function and antiviral mechanism.
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