Inhibition Effect of C8orf4 Gene Encoding Protein on in vitro Replication of Porcine Epidemic Diarrhea Virus
C8orf4,also known as thyroid carcinoma 1(TC1),was originally cloned from thyroid carcinoma and its surrounding normal thyroid tissue.C8orf4 is universally expressed in vertebrates with sequence conservation across species.Previous studies have shown that C8orf4 is highly expressed in tumors and is involved in various cancer cell communication.It is a novel regulator of cancer and inflammation,and enhances inflammation by regulating NF-κB transcription.However,little is known about the effects of C8orf4 on viruses.This experiment was designed to study the effect of C8orf4 encoding protein on the replication of porcine epidemic diarrhea virus(PEDV).PEDV was inoculated into Vero cells,and the effect of PEDV on C8orf4 expression was detected by qRT-PCR and Western blot at different time points.The eukaryotic expression vector pcDNA3.1-C8orf4-His and specific siRNA of C8orf4 gene were designed and synthesized.The effect of C8orf4 on PEDV replication was detected by qRT-PCR and Western blot through overexpression and inhibition of C8orf4 expression.Furthermore,the effect of C8orf4 encoding protein on the proliferation of PEDV at each stage of the replication cycle was determined by overexpression of C8orf4 encoding protein.With the extension of virus infection time,the expression of C8orf4 in cells showed an upward trend.Overexpression of C8orf4 significantly inhibited PEDV replication,while interfered with C8orf4 expression to promote PEDV replication,and C8orf 4 encoding protein mainly played a role in the biosynthetic phase of PEDV replication cycle.This study proved that C8orf 4 encoding protein can inhibit PEDV replication,which provides a reference for the study of the function of C8orf4 and the basis for the study of anti-PEDV replication mechanism.
国家科技期刊平台
NSTL
万方数据
