猫疱疹病毒1型的检测及一株分离毒株的致病性
Detection of Feline Herpesvirus Type 1 and Pathogenicity of an Isolated Strain
邓梏男 1张家祺 1保志鹏 2陈涛云 1喻琦胜 1丁露 1朱晨曦 1王怡 1任玉鹏 3贺超 4张斌3
作者信息
- 1. 西南民族大学畜牧兽医学院,成都 610041
- 2. 云南农业职业技术学院,昆明 650212
- 3. 西南民族大学畜牧兽医学院,成都 610041;青藏高原动物遗传资源保护与利用教育部/四川省重点实验室,成都 610041
- 4. 四川省兽药监察所,成都 610041
- 折叠
摘要
旨在调查成都地区的猫疱疹病毒1型(feline herpesvirus-1,FHV-1)的流行及遗传变异情况.试验选取2020-2023年从成都地区采集的178份具有呼吸道症状猫的眼、鼻及口咽拭子进行FHV-1核酸检测、gE基因遗传进化分析、FHV-1的分离鉴定和致病性研究.结果发现,成都地区FHV-1阳性率为11.8％(21/178);其中宠物医院FHV-1阳性率为6.4％(7/110),猫舍FHV-1阳性率为20.6％(14/68),表明在猫舍中FHV-1感染率更高.FHV-1 gE基因的遗传进化分析结果表明,本研究扩增出的5株gE基因和疫苗株的核苷酸同源性在99.1％～100％,并发现PX-9株存在3个独特的氨基酸突变位点(L259Q、C294I、W295F).通过CRFK细胞对5份阳性样本进行病毒的分离培养,结果PX-9毒株被成功分离鉴定.对PX-9毒株进行动物回归试验,结果表明攻毒组猫只表现出眼鼻分泌物增多,打喷嚏等临床症状,在接毒后第4天病毒拷贝数最高,为9.05 × 107 copies·μL-1,且排毒期较长;攻毒猫只FHV-1病毒载量检测结果显示,气管及肺组织病毒拷贝数分别为1.7×107和3.5 × 104 copies·μg-1,其它组织核酸阴性.综上表明,在成都地区仍然存在FHV-1流行,并且流行毒株有较强的致病性.
Abstract
The aim of this study was to investigate the prevalence and genetic diversity of feline herpesvirus-1(FHV-1)in Chengdu city.From 2020 to 2023,a total of 178 conjunctiva,pharynx and nose swabs were collected from cats with respiratory symptoms in Chengdu city.The collected samples were subjected to FHV-1 PCR detection,gE gene genetic evolutionary analysis,viral isolation and identification,as well as pathogenicity.The results showed that,the FHV-1 positivity rate in Chengdu city was 11.8％(21/178).In which the FHV-1 positivity rate of veterinary hospitals was 6.4％(7/110)and 20.6％(14/68)in catteries,suggested that the higher rate of FHV-1 infection was in catteries.The genetic evolutionary analysis of five FHV-1 gE genes showed that the nucleotide homology was 99.1％～100％to the vaccine strains,and three unique amino acid mutation sites were found in PX-9 strain(L259Q,C294I,W295F).Five positive samples were isolated and cultured by CRFK cell,only PX-9 strain was successfully isolated and identified from CRFK cells.The results of animal regression experiment by PX-9 strain showed that all cats in the infected group exhibited clinical symptoms,such as increased ocular,nasal secretions and sneezing.The highest viral load was found on the 4th day after inoculation(9.05×107 copies·μL-1),and the detoxification period is relatively long.The results of FHV-1 viral load were 1.7 × 107 and 3.5 × 104 copies·μg-1 in trachea and lungs of infected group,respectively.The other tissues were negative for FHV-1 DNA.The results of this study indicated that FHV-1 was still prevalent in Chengdu and the prevalent strains had highly pathogenicity.
关键词
FHV-1/流行情况/遗传变异/动物回归试验Key words
FHV-1/prevalence/genetic variation/animal regression experiment引用本文复制引用
基金项目
国家农业产业技术体系建设项目四川兽药创新团队项目(SCCXTD-2020-18)
西南民族大学人才支持基金(RQD2021004)
出版年
2024
国家科技期刊平台
NSTL
万方数据