Influence of Meat Sheep Varieties on the Scale Application of in vitro Embryo Production Technology
The aim of this study was to explore the impact of breed factors on the efficiency of commercial rapid propagation of OPU-IVEP.This study used healthy 8-month-old to 3-year-old black headed Suffolk,black headed Dorper,and Texel meat breeding ewes identified by breed identification as donors.OPU technology was used to obtain oocytes at Inner Mongolia Saino Sheep Technology Co.,Ltd.for in vitro embryo factory production or in vivo embryos obtained through laparoscopic insemination and surgical flushing.Embryo transfer was performed,and relevant data of oocyte recovery,embryo development,and transfer pregnancy were recorded for statistical analysis.The differences in oocyte recovery efficiency,oocyte in vitro fertilization and embryo development ability,embryo transfer pregnancy and lambing efficiency among different breeds in large-scale in vitro embryo production was compared;At the same time,the applica-tion effects of in vivo and in vitro embryo production technologies of different breeds were also compared.The results showed that:1)there was a significant difference in oocyte recovery rates among the 3 breeds(P＜0.01),with the highest recovery efficiency observed in the black headed Dorper;2)There was no significant difference in the in vitro fertilization and cleavage rates among the 3 breeds(P＞0.05),but the blastocyst development rate of Texel sheep was signifi-cantly higher than the other 2 breeds(P＜0.01);3)There was no significant difference(P＞0.05)in the conception rate and average number of lambs produced by embryo transfer between different breeds,whether in vivo or in vitro,and there was no significant difference(P＞0.05)in the average conception rate and lambing rate of in vivo and in vitro embryo transfer in different breeds.In summary,in the process of OPU-IVEP industrial and large-scale application in sheep in vitro embryo production,there are differences in the efficiency among different breeds,such as follicle development,number of retrieved oocytes,and blastocyst development rate.Texel sheep,which has the lowest average number of follicles and average number of retrieved oocytes,has the highest blastocyst development rate;The conception and lambing situation after transplantation,which directly affects the efficiency of rapid reproduction,did not show significant differences among different varieties,and there was no significant difference in the number of offspring obtained from single production of in vivo and in vitro embryos.However,the donor utilization rate of OPU-IVEP is higher than that of MOET.
万方数据
维普
