Combination of IGF1,CoQ10 and MT Alleviated the Effects of Heat Stress on Bovine IVF Blastocysts
The aim of this study was to investigate the effects of combination of insulin-like growth factor 1(IGF1),coenzyme Q10(CoQ10),and melatonin(MT)on development of bo-vine oocytes,embryos and heat stressed blastocysts.In this study,bovine ovaries were collected from the abattoir and cumulus-oocyte complexes(COCs)were extracted in the laboratory.IGF1,CoQ10 and MT were added to bovine oocyte in vitro maturation(IVM)solution and bovine embryo in vitro culture(IVC)solution.The developmental ability,level of reactive oxygen spe-cies(ROS)and mitochondrial membrane potential(△Ψm)of bovine oocytes from each treatment were detected.By applying 41 ℃ heat stress at the blastocyst stage,the effects of heat stress and combined supplementation on the developmental ability and apoptosis level of bovine IVF blasto-cysts were determined,and the mRNA expression levels of embryo quality related genes were de-tected by qRT-PCR.Each experimental group was repeated 3 times.The results showed that the combination of IGF1,CoQ10 and MT(ICM group)significantly increased oocyte maturation rate((90.40±2.06)％vs.(65.41±0.63)％),cleavage rate((93.33±1.96)％vs.(59.77±2.93)％)and blastocyst rate((51.43±5.34)％vs.(26.92±3.24)％))(P＜0.05)compared with the non added group(CT-0 group).In the ICM group,the ROS level of bovine oocytes was significantly decreased and the △Ψm of bovine oocytes was significantly increased(P＜0.05).Compared with the heat stress group(HS group),the combination of IGF1,CoQ10 and MT(ICM+HS group)significantly increased expanding rate((62.00±2.97)％vs.(30.77±8.66)％,P＜0.05)of heat stressed blastocysts,inhibited apoptosis of heat stressed blastocyst cells,and increased mRNA expression levels of IGFBP3,ATP1A1,DSC2,and IFNT2(P＜0.05)of heat stressed blastocysts.In summary,the combination of IGF1,CoQ10 and MT can effectively enhance the developmental ability,reduce the ROS level and increase the △Ψm of bo-vine oocytes.Heat stress reduced the expanding rate of bovine blastocysts,promoted apoptosis of bovine blastocyst cells and damaged the quality of blastocysts,while the combination of IGF1,CoQ10 and MT alleviated the damage from heat stress.
万方数据
维普
