Pathogenicity and Genomic Characteristics of Feline Panleukopenia Virus A91S Variant in Cats
Feline panleukopenia virus(FPV)is a highly pathogenic threat to cats.In recent years,an FPV variant harboring an amino acid mutation at the VP2 91 site(A to S)has emerged promi-nently in China,however,information regarding on its pathogenicity is lacking.The aim of this study was to investigate the infection and molecular characteristics of the FPV A91S variant.F81 cells were used to isolate the FPV A91S variant,and the hemagglutination properties,pathoge-nicity and genomic characteristics were determined.An FPV A91S variant was successfully isola-ted,with a virus titer of 104.6 TCID50·mL-1 after viral purification.This isolate could effectively agglutinate porcine erythrocytes at pH 6.0-6.5 and temperatures of 4 ℃ and 37 ℃.When orally administered to kittens,the isolate caused a spectrum of clinical manifestations including vomi-ting,diarrhea,severe leukopenia,purulent discharge from the eyes,and cat death within 5 days.Gross and histopathological examinations unveiled severe hemorrhaging in the intestines and lungs.The nearly full genome sequence of this isolate was obtained,and the key amino acid resi-dues were consistent with those of the typical FPV.In addition to the A91S mutation in VP2,three distinctive amino acid mutations(D23N,I443V and H596Q)were observed in the NS1 pro-tein.These amino acid mutations caused the FPV A91S strains to form a discrete branch on all the evolutionary trees.This study,for the first time,investigated the hemagglutination proper-ties and pathogenicity of the FPV A91S variant,which contribute valuable insights into the bio-logical characteristics and genetic variations of FPV.
feline panleukopenia virusVP2 A91 S varianthemagglutinationpathogenicityge-netic evolution
万方数据
维普
